| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Bandara, Aloka B. Ramachandran, Siddharth Zuo, Ziwei Inzana, Thomas J. Ritter, Alfred Heflin, James R. |
| Description |
Author Affiliation: Bandara AB ( Department of Biomedical Sciences and Pathobiology, Life Sciences 1, 970 Washington Street, SW, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address: abandara@vt.edu.); Zuo Z ( Department of Physics, 850 West Campus Drive, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address: zuo@vt.edu.); Ramachandran S ( Department of Electrical and Computer Engineering, 8 Saint Mary's Street, Boston University, Boston, MA 02215, USA. Electronic address: sidr@bu.edu.); Ritter A ( Virginia nanoTech, LLC, 2200 Kraft Drive, Blacksburg, VA 24060, USA. Electronic address: rittera@vnanot.com.); Heflin JR ( Department of Physics, 850 West Campus Drive, Virginia Tech, Blacksburg, VA 24061, USA); Inzana TJ ( Department of Biomedical Sciences and Pathobiology, Life Sciences 1, 970 Washington Street, SW, Virginia Tech, Blacksburg, VA 24061, USA) |
| Abstract |
Methicillin-resistance among Staphylococcus species is a major health problem in hospitals, communities, and animals. There is a need for culture-free diagnostic assays that can be carried out rapidly, and maintain a high degree of sensitivity and specificity. To address this need an ionic self-assembled multilayer (ISAM) film was deposited on the surface of a long-period grating (LPG) optical fiber by immersion alternately in poly-allylamine hydrochloride and in poly-1-[p-(3'-carboxy-4'-hydroxyphenylazo) benzenesulfonamido]-1,2-ethandiyl (PCBS), resulting in terminal carboxyl groups on the LPG-ISAM. The terminal carboxyl groups were covalently conjugated to monoclonal antibodies (MAb) specific to penicillin-binding-protein 2a of methicillin resistant (MR) staphylococci. After exposure of the LPG-ISAM to 10(2) colony forming units (CFU)/ml of MR S. aureus (MRSA) for 50 min., light transmission was reduced by 19.7%. In contrast, after exposure to 10(6) CFU/ml of methicillin-sensitive S. aureus (MSSA) attenuation of light transmission was less than 1.8%. Exposure of the LPG-ISAM to extracts of liver, lungs, or spleen from mice infected with MRSA attenuated light transmission by 11.7-73.5%. In contrast, exposure of the biosensor to extracts from MSSA-infected mice resulted in 5.6% or less attenuation of light transmission. When the sensor was tested with 36 strains of MR staphylococci, 15 strains of methicillin-sensitive staphylococci, 10 strains of heterologous genera (all at 10(4) CFU/ml), or tissue samples from mice infected with MRSA, there was complete agreement between MR and non-MR bacteria determined by antibiotic susceptibility testing and the biosensor assay when the cutoff value for attenuation of light transmission was 6.3%. Thus, the biosensor described has the potential to detect MR staphylococci in clinical samples with a high degree of sensitivity and specificity. |
| ISSN |
09565663 |
| Volume Number |
70 |
| e-ISSN |
18734235 |
| Journal |
Biosensors and Bioelectronics |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-08-15 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Bacterial Load Instrumentation Biosensing Techniques Fiber Optic Technology Methicillin-resistant Staphylococcus Aureus Isolation & Purification Microarray Analysis Refractometry Equipment Design Equipment Failure Analysis Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. Discipline Biotechnology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
