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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Beni, Valerio Ali, Mounir Ben Turner, Anthony P. F. Azzouzi, Sawsen Mak, Wing Cheung Kor, Kamalodin |
| Description | Country affiliation: Tunisia Author Affiliation: Azzouzi S ( Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden); Mak WC ( Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden. Electronic address: wing.cheung.mak@liu.se.); Kor K ( Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden); Turner AP ( Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden.); Ali MB ( University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, Cité Ettafala, 4003 Ibn Khaldoun Sousse, Tunisia.); Beni V ( Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden. Electronic address: valerio.beni@acreo.se.) |
| Abstract | Alteration in expression of miRNAs has been correlated with different cancer types, tumour stage and response to treatments. In this context, a structurally responsive oligonucleotide-based electrochemical impedimetric biosensor has been developed for the simple and sensitive detection of miRNA-21. A highly specific biotinylated DNA/LNA molecular beacon (MB) probe was conjugated with gold nanoparticles (AuNPs) to create an integrated, dual function bio-label (biotin-MB-AuNPs) for both biorecognition and signal generation. In the presence of target miRNA-21, hybridisation takes place resulting in the 'activation' of the biotin-MB; this event makes the biotin group, which was previously 'protected' by the steric hindrance of the MB stem-loop structure, accessible. The activated biotin-MB-AuNPs/miRNA complexes become available for capture, via supramolecular interaction, onto a nentravidin-modified electrode for electrochemical transduction. The binding event results in a decrease of the charge transfer resistance at the working electrode/electrolyte interface. The biosensor responded linearly in the range 1-1000 pM of miRNA-21, with a limit of detection of 0.3 pM, good reproducibility (Relative Standard deviation (RSD) =3.3%) and high selectivity over other miRNAs (i.e. miRNA-221 and miRNA-205) sequences. Detection of miRNA-21 in spiked serum samples at clinically relevant levels (low pM range) was also demonstrated, thus illustrating the potential of the biosensor for point-of-care clinical applications. The proposed biosensor design, based on the combination of a neutravidin transducing surface and the dual-function biotin-MB-AuNPs bio-label, provides a simple and robust approach for detection of short-length nucleic acid targets, such as miRNAs. |
| ISSN | 09565663 |
| Journal | Biosensors and Bioelectronics |
| Volume Number | 92 |
| e-ISSN | 18734235 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-06-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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