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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Crossland, J. Clark, C. C. Martinez-hernandez, A. Kaplan, G. |
| Abstract | The collagens associated with 14.5-d rat visceral yolk sacs were localized and identified by a variety of procedures. Morphological examination showed that both the visceral epithelium and mesothelium rested upon thin basement membranes, whereas the majority of the extracellular matrix consisted of a stroma containing occasional cells and abundant banded fibrils. Immunohistochemistry at the electron microscope level showed that the basement membranes specifically cross- reacted with antibodies directed against mouse basement membrane components, whereas the stroma specifically cross-reacted with antibodies directed against rat type I collagen. Extractions of acellular visceral yolk sacs and subsequent analyses showed that type I collagen components were prevalent. Furthermore, in vitro biosynthetic studies showed only the presence of type I procollagen components (or their conversion products) and alpha-fetoprotein. These findings, taken together with our previous studies on the 14.5-d rat parietal yolk sac, provide us with protein markers for studying the origin of cells in rat parietovisceral yolk sac carcinomas. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 93 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1982-05-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Collagen Connective Tissue Yolk Sac Animals Basement Membrane Isolation & Purification Epithelium Ultrastructure Membranes Microscopy, Electron Procollagen Rats, Inbred Strains Metabolism Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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