NDLI: Inhibition of synthesis of alpha-fetoprotein by glucocorticoids in cultured hepatoma cells

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chou, J. Y. Feldman, M. Mano, T.
Abstract	alpha-Fetoprotein (AFP) synthesis was studied in the presence and absence of glucocorticoids in rat hepatoma Mc-A-RH-7777 cells. Radioimmunoassay of media from cell cultures grown in the presence of glucocorticoid (dexamethasone or cortisol) showed a reduction in AFP, an increase in albumin, and no significant change in transferrin accumulation, as compared to controls. Labeling experiments with L- [35S]methionine indicated that in both cells and media of dexamethasone- treated cultures there was a 50--80% reduction in polypeptide precipitated by anti-AFP serum, as compared with controls; no change was seen in polypeptide precipitated by anti-transferrin serum. Pulse and pulse-chase experiments demonstrated that dexamethasone inhibited the synthesis of AFP but not its secretion. The half-time for secretion of AFP in the presence and absence of dexamethasone was 43 min.
ISSN	00219525
e-ISSN	15408140
Journal	The Journal of Cell Biology
Issue Number	2
Volume Number	93
Language	English
Publisher	Rockefeller University Press (United States)
Publisher Date	1982-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Dexamethasone Pharmacology Hydrocortisone Alpha-Fetoproteins Biosynthesis Albumins Animals Cell Line Kinetics Liver Neoplasms, Experimental Methionine Metabolism Transferrin Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine

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Inhibition of synthesis of alpha-fetoprotein by glucocorticoids in cultured hepatoma cells