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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Uchida, T. Mekada, E. Okada, Y. |
| Description | Author Affiliation: Mekada E ( Institute for Molecular and Cellular Biology, Osaka University, Japan.) |
| Abstract | Two substances possessing the ability to bind to diphtheria toxin (DT) were found to be present in a membrane fraction from DT-sensitive Vero cells. One of these substances was found on the basis of its ability to bind DT and inhibit its cytotoxic effect. This inhibitory substance competitively inhibited the binding of DT to Vero cells. However this inhibitor could not bind to CRM197, the product of a missense mutation in the DT gene, and did not inhibit the binding of CRM197 to Vero cells. Moreover, similar levels of the inhibitory activity were observed in membrane fractions from DT-insensitive mouse cells, suggesting the inhibitor is not the DT receptor which is specifically present in DT-sensitive cells. The second DT-binding substance was found in the same Vero cell membrane preparation by assaying the binding of 125I-labeled CRM197. Such DT-binding activity could not be observed in membrane preparation from mouse L cells. From competition studies using labeled DT and CRM proteins, we conclude that this binding activity is due to the surface receptor for DT. Treatment of these substances with several enzymes revealed that the inhibitor was sensitive to certain RNases but resistant to proteases, whereas the DT receptor was resistant to RNase but sensitive to proteases. The receptor was solubilized and partially purified by chromatography on CM- Sepharose column. Immunoprecipitation and Western blotting analysis of the partially purified receptor revealed that a 14.5-kD protein is the DT receptor, or at least a component of it. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 107 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1988-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Diphtheria Toxin Metabolism Membrane Proteins Receptors, Cell Surface Receptors, Cholinergic Animals Bacterial Proteins Binding, Competitive Cell Membrane Chromatography, Gel Chromatography, Ion Exchange Cross Reactions Electrophoresis, Polyacrylamide Gel Heparin-binding EGF-like Growth Factor Immunoassay Intercellular Signaling Peptides And Proteins L Cells (Cell Line) Precipitin Tests Vero Cells Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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