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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Infante, C. Bornens, M. Ramos-morales, F. Rios, R. M. Fedriani, C. |
| Description | Author Affiliation: Infante C ( Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla Apdo. 1095, 41080-Sevilla, Spain.) |
| Abstract | We report that a peripheral Golgi protein with a molecular mass of 210 kD localized at the cis-Golgi network (Rios, R.M., A.M. Tassin, C. Celati, C. Antony, M.C. Boissier, J.C. Homberg, and M. Bornens. 1994. J. Cell Biol. 125:997–1013) is a microtubule-binding protein that associates in situ with a subpopulation of stable microtubules. Interaction of this protein, now called GMAP-210, for Golgi microtubule-associated protein 210, with microtubules in vitro is direct, tight and nucleotide-independent. Biochemical analysis further suggests that GMAP-210 specifically binds to microtubule ends. The full-length cDNA encoding GMAP-210 predicts a protein of 1,979 amino acids with a very long central coiled-coil domain. Deletion analyses in vitro show that the COOH terminus of GMAP-210 binds to microtubules whereas the $NH_{2}$ terminus binds to Golgi membranes. Overexpression of GMAP-210–encoding cDNA induced a dramatic enlargement of the Golgi apparatus and perturbations in the microtubule network. These effects did not occur when a mutant lacking the COOH-terminal domain was expressed. When transfected in fusion with the green fluorescent protein, the $NH_{2}-terminal$ domain associated with the cis-Golgi network whereas the COOH-terminal microtubule-binding domain localized at the centrosome. Altogether these data support the view that GMAP-210 serves to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. In addition, this interaction appears essential for ensuring the proper morphology and size of the Golgi apparatus. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 1 |
| Volume Number | 145 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1999-04-05 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Centrosome Metabolism Golgi Apparatus Microtubule-Associated Proteins Isolation & Purification Microtubules Amino Acid Sequence Animals Binding Sites COS Cells DNA, Complementary Genetics Genes, Reporter Ultrastructure Green Fluorescent Proteins HeLa Cells Interphase Luminescent Proteins Microscopy, Fluorescence Physiology Molecular Motor Proteins Molecular Sequence Data Molecular Weight Nuclear Proteins Paclitaxel Pharmacology Recombinant Fusion Proteins Sequence Deletion Transfection Tubulin Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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