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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mavroidis, M. Capetanaki, Y. Milner, D. J. Weisleder, N. |
| Description | Author Affiliation: Milner DJ ( Department of Molecular and Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.) |
| Abstract | Ultrastructural studies have previously suggested potential association of intermediate filaments (IFs) with mitochondria. Thus, we have investigated mitochondrial distribution and function in muscle lacking the IF protein desmin. Immunostaining of skeletal muscle tissue sections, as well as histochemical staining for the mitochondrial marker enzymes cytochrome C oxidase and succinate dehydrogenase, demonstrate abnormal accumulation of subsarcolemmal clumps of mitochondria in predominantly slow twitch skeletal muscle of desmin-null mice. Ultrastructural observation of desmin-null cardiac muscle demonstrates in addition to clumping, extensive mitochondrial proliferation in a significant fraction of the myocytes, particularly after work overload. These alterations are frequently associated with swelling and degeneration of the mitochondrial matrix. Mitochondrial abnormalities can be detected very early, before other structural defects become obvious. To investigate related changes in mitochondrial function, we have analyzed ADP-stimulated respiration of isolated muscle mitochondria, and ADP-stimulated mitochondrial respiration in situ using saponin skinned muscle fibers. The in vitro maximal rates of respiration in isolated cardiac mitochondria from desmin-null and wild-type mice were similar. However, mitochondrial respiration in situ is significantly altered in desmin-null muscle. Both the maximal rate of ADP-stimulated oxygen consumption and the dissociation constant $(K_{m})$ for ADP are significantly reduced in desmin-null cardiac and soleus muscle compared with controls. Respiratory parameters for desmin-null fast twitch gastrocnemius muscle were unaffected. Additionally, respiratory measurements in the presence of creatine indicate that coupling of creatine kinase and the adenine translocator is lost in desmin-null soleus muscle. This coupling is unaffected in cardiac muscle from desmin-null animals. All of these studies indicate that desmin IFs play a significant role in mitochondrial positioning and respiratory function in cardiac and skeletal muscle. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 6 |
| Volume Number | 150 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2000-09-18 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Respiration Physiology Desmin Genetics Intermediate Filaments Metabolism Mitochondria Myocardium Adenosine Diphosphate Pharmacology Animals Cardiomyopathies Energy Metabolism Drug Effects Ultrastructure Mice Mice, Knockout Microscopy, Electron Muscle Fibers, Skeletal Muscle, Skeletal Cytology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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