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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Webb, D. J. Laukaitis, C. M. Donais, K. Horwitz, A. F. |
| Description | Author Affiliation: Laukaitis CM ( Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, USA.) |
| Abstract | To investigate the mechanisms by which adhesions form and disperse in migrating cells, we expressed α5 integrin, α-actinin, and paxillin as green fluorescent protein (GFP) fusions. All localized with their endogenous counterparts and did not perturb migration when expressed at moderate levels. α5-GFP also rescued the adhesive defects in CHO B2 cells, which are α5 integrin deficient. In ruffling cells, α5-GFP and α-actinin–GFP localized prominently at the leading edge in membrane protrusions. Of the three GFP fusion proteins that we examined, paxillin was the first component to appear visibly organized in protrusive regions of the cell. When a new protrusion formed, the paxillin appeared to remodel from older to newer adhesions at the leading edge. α-Actinin subsequently entered adhesions, which translocated toward the cell center, and inhibited paxillin turnover. The new adhesions formed from small foci of α-actinin–GFP and paxillin-GFP, which grew in size. Subsequently, α5 integrin entered the adhesions to form visible complexes, which served to stabilize the adhesions. α5-GFP also resided in endocytic vesicles that emanated from the leading edge of protrusions. Integrin vesicles at the cell rear moved toward the cell body. As cells migrated, α5 vesicles also moved from a perinuclear region to the base of the lamellipodium. The α5 vesicles colocalized with transferrin receptor and FM 4-64 dye. After adhesions broke down in the rear, α5-GFP was found in fibrous structures behind the cell, whereas α-actinin–GFP and paxillin-GFP moved up the lateral edge of retracting cells as organized structures and then dissipated. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 7 |
| Volume Number | 153 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2001-06-25 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Actinin Metabolism Antigens, CD Cell Movement Physiology Cytoskeletal Proteins Phosphoproteins Genetics Animals Pharmacology CHO Cells Cell Adhesion Drug Effects Cell Line Cell Membrane Structures Cricetinae Cytoskeleton Fluorescent Dyes Green Fluorescent Proteins Integrin Alpha5 Intracellular Fluid Luminescent Proteins Macromolecular Substances Microscopy, Video Paxillin Protein Transport Pseudopodia Ultrastructure Receptors, Transferrin Recombinant Fusion Proteins Transport Vesicles Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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