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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fuller, R. S. Sipos, G. Brickner, J. H. Blanchette, J. M. |
| Description | Author Affiliation: Brickner JH ( Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.) |
| Abstract | Using a new assay for membrane fusion between late Golgi/endosomal compartments, we have reconstituted a rapid, robust homotypic fusion reaction between membranes containing Kex2p and Ste13p, two enzymes resident in the yeast trans-Golgi network (TGN). Fusion was temperature, ATP, and cytosol dependent. It was inhibited by dilution, $Ca^{+2}$ chelation, N-ethylmaleimide, and detergent. Coimmunoisolation confirmed that the reaction resulted in cointegration of the two enzymes into the same bilayer. Antibody inhibition experiments coupled with antigen competition indicated a requirement for soluble NSF attachment protein receptor (SNARE) proteins Tlg1p, Tlg2p, and Vti1p in this reaction. Membrane fusion also required the rab protein Vps21p. Vps21p was sufficient if present on either the Kex2p or Ste13p membranes alone, indicative of an inherent symmetry in the reaction. These results identify roles for a Tlg SNARE complex composed of Tlg1p, Tlg2p, Vti1p, and the rab Vps21p in this previously uncharacterized homotypic TGN fusion reaction. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 6 |
| Volume Number | 155 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2001-12-10 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Carrier Proteins Metabolism Membrane Fusion Physiology Membrane Proteins Genetics Membrane Transport Proteins Saccharomyces Cerevisiae Proteins Vesicular Transport Proteins Trans-Golgi Network Cell-Free System Fucosyltransferases Qa-SNARE Proteins Qb-SNARE Proteins SNARE Proteins Yeasts Rab GTP-Binding Proteins Rab5 GTP-Binding Proteins Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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