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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yates, John R. Drubin, David G. Barnes, Georjana Anderson, Scott Westermann, Stefan Cheeseman, Iain M. |
| Description | Author Affiliation: Westermann S ( Department of Molecular and Cell Biology, Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA. gbarnes@socrates.berkeley.edu) |
| Abstract | How kinetochore proteins are organized to connect chromosomes to spindle microtubules, and whether any structural and organizational themes are common to kinetochores from distantly related organisms, are key unanswered questions. Here, we used affinity chromatography and mass spectrometry to generate a map of kinetochore protein interactions. The budding yeast CENP-C homologue Mif2p specifically copurified with histones H2A, H2B, and H4, and with the histone H3-like CENP-A homologue Cse4p, strongly suggesting that Cse4p replaces histone H3 in a specialized centromeric nucleosome. A novel four-protein Mtw1 complex, the Nnf1p subunit of which has homology to the vertebrate kinetochore protein CENP-H, also copurified with Mif2p and a variety of central kinetochore proteins. We show that Mif2 is a critical in vivo target of the Aurora kinase Ipl1p. Chromatin immunoprecipitation studies demonstrated the biological relevance of these associations. We propose that a molecular core consisting of CENP-A, -C, -H, and Ndc80/HEC has been conserved from yeast to humans to link centromeres to spindle microtubules. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 163 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2003-10-27 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Kinetochores Chemistry Saccharomycetales Amino Acid Sequence Aurora Kinases Cell Cycle Proteins Isolation & Purification Metabolism Centromere Chromatin Chromosomal Proteins, Non-Histone Conserved Sequence DNA-Binding Proteins Genetics Fungal Proteins Histones Models, Biological Molecular Sequence Data Mutagenesis, Site-Directed Nucleosomes Phosphorylation Protein Subunits Protein-Serine-Threonine Kinases Saccharomyces Cerevisiae Proteins Sequence Homology, Amino Acid Spindle Apparatus Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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