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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Greer, Yoshimi Endo Rubin, Jeffrey S. |
| Description | Author Affiliation: Greer YE ( Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.) |
| Abstract | Previously we determined that Dishevelled-2/3 (Dvl) mediate Wnt-3a–dependent neurite outgrowth in Ewing sarcoma family tumor cells. Here we report that neurite extension was associated with Dvl phosphorylation and that both were inhibited by the casein kinase 1 (CK1) δ/ε inhibitor IC261. Small interfering RNAs targeting either CK1δ or CK1ε decreased Dvl phosphorylation, but only knockdown of CK1δ blocked neurite outgrowth. CK1δ but not CK1ε was detected at the centrosome, an organelle associated with neurite formation. Deletion analysis mapped the centrosomal localization signal (CLS) of CK1δ to its C-terminal domain. A fusion protein containing the CLS and EGFP displaced full-length CK1δ from the centrosome and inhibited Wnt-3a–dependent neurite outgrowth. In contrast to wild-type CK1ε, a chimera comprised of the kinase domain of CK1ε and the CLS of CK1δ localized to the centrosome and rescued Wnt-3a–dependent neurite outgrowth suppressed by CK1δ knockdown. These results provide strong evidence that the centrosomal localization of CK1δ is required for Wnt-3a–dependent neuritogenesis. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 6 |
| Volume Number | 192 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2011-03-21 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Casein Kinase Idelta Metabolism Centrosome Isoenzymes Neurites Physiology Wnt Proteins Adaptor Proteins, Signal Transducing Genetics Animals Antagonists & Inhibitors Casein Kinase Iepsilon Cell Line DNA Mutational Analysis Mice Phosphoproteins RNA, Small Interfering Recombinant Fusion Proteins Wnt3 Protein Wnt3A Protein Research Support, N.I.H., Intramural Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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