NDLI: Structural basis of transcription activation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Feng, Yu Ebright, Richard H. Zhang, Yu
Description	Author Affiliation: Feng Y ( Waksman Institute and Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.); Zhang Y ( Waksman Institute and Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.); Ebright RH ( Waksman Institute and Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA. ebright@waksman.rutgers.edu.)
Abstract	Class II transcription activators function by binding to a DNA site overlapping a core promoter and stimulating isomerization of an initial RNA polymerase (RNAP)-promoter closed complex into a catalytically competent RNAP-promoter open complex. Here, we report a 4.4 angstrom crystal structure of an intact bacterial class II transcription activation complex. The structure comprises Thermus thermophilus transcription activator protein TTHB099 (TAP) [homolog of Escherichia coli catabolite activator protein (CAP)], T. thermophilus RNAP σ(A) holoenzyme, a class II TAP-dependent promoter, and a ribotetranucleotide primer. The structure reveals the interactions between RNAP holoenzyme and DNA responsible for transcription initiation and reveals the interactions between TAP and RNAP holoenzyme responsible for transcription activation. The structure indicates that TAP stimulates isomerization through simple, adhesive, stabilizing protein-protein interactions with RNAP holoenzyme.
ISSN	00368075
Issue Number	6291
Volume Number	352
e-ISSN	10959203
Journal	Science
Language	English
Publisher	American Association for the Advancement of Science (United States)
Publisher Date	2016-06-10
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacterial Proteins Chemistry Cyclic AMP Receptor Protein DNA, Bacterial DNA-Directed RNA Polymerases Gene Expression Regulation, Bacterial Sigma Factor Transcriptional Activation Ultrastructure Crystallography, X-Ray Holoenzymes Promoter Regions, Genetic Protein Conformation Thermus Thermophilus Enzymology Genetics Journal Article Research Support, N.I.H., Extramural Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	History and Philosophy of Science

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