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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wu, Yimin Li, Tong Gluhak-heinrich, Jelica Martinez, Marcos Chen, Lei Chen, Shuo Chuang, Hui-hsiu Dong, Juan Gay, Isabel Macdougall, Mary |
| Description | Author Affiliation: Chen S ( Department of Pediatric Dentistry, University of Texas Health Science Center, San Antonio, Texas 78229-3900, USA.) |
| Abstract | Dentin sialophosphoprotein (DSPP), an important odontoblast differentiation marker, is necessary for tooth development and mineralization. Bone morphogenetic protein 2 (BMP2) plays a vital role in odontoblast function via diverse signal transduction systems. We hypothesize that BMP2 regulates DSPP gene transcription and thus odontoblast differentiation. Here we report that expression of BMP2 and DSPP is detected during mouse odontogenesis by in situ hybridization assay, and BMP2 up-regulates DSPP mRNA and protein expression as well as DSPP-luciferase promoter activity in mouse preodontoblasts. By sequentially deleting fragments of the mouse DSPP promoter, we show that a BMP2-response element is located between nucleotides -97 and -72. By using antibody and oligonucleotide competition assays in electrophoretic mobility shift analysis and chromatin immunoprecipitation experiments, we show that the heterotrimeric transcription factor Y (NF-Y) complex physically interacts with the inverted CCAAT box within the BMP2-response element. BMP2 induces NF-Y accumulation into the nucleus increasing its recruitment to the mouse DSPP promoter in vivo. Furthermore, forced overexpression of NF-Y enhances promoter activity and increases endogenous DSPP protein levels. In contrast, mutations in the NF-Y-binding motif reduce BMP2-induced DSPP transcription. Moreover, inhibiting BMP2 signaling by Noggin, a BMP2 antagonist, results in significant inhibition of DSPP gene expression in preodontoblasts. Taken together, these results indicate that BMP2 mediates DSPP gene expression and odontoblast differentiation via NF-Y signaling during tooth development. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 28 |
| Volume Number | 283 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2008-07-11 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bone Morphogenetic Proteins Metabolism CCAAT-Binding Factor Cell Differentiation Physiology Gene Expression Regulation, Developmental Odontoblasts Odontogenesis Protein Precursors Biosynthesis Signal Transduction Transforming Growth Factor Beta Amino Acid Motifs Animals Bone Morphogenetic Protein 2 Genetics Carrier Proteins Cell Line Cell Nucleus Extracellular Matrix Proteins Mice Mice, Inbred ICR Cytology Phosphoproteins RNA, Messenger Response Elements Sequence Deletion Sialoglycoproteins Transcription, Genetic Up-Regulation Research Support, N.I.H., Extramural Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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