NDLI: Very early reaction intermediates detected by microsecond time scale kinetics of cytochrome cd1-catalyzed reduction of nitrite.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ferguson, Stuart J. De Vries, Simon Strampraad, Marc J. F. Sam, Katharine A.
Description	Author Affiliation: Sam KA ( Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.)
Abstract	Paracoccus pantotrophus cytochrome cd(1) is a nitrite reductase found in the periplasm of many denitrifying bacteria. It catalyzes the reduction of nitrite to nitric oxide during the denitrification part of the biological nitrogen cycle. Previous studies of early millisecond intermediates in the nitrite reduction reaction have shown, by comparison with pH 7.0, that at the optimum pH, approximately pH 6, the earliest intermediates were lost in the dead time of the instrument. Access to early time points (approximately 100 micros) through use of an ultra-rapid mixing device has identified a spectroscopically novel intermediate, assigned as the Michaelis complex, formed from reaction of fully reduced enzyme with nitrite. Spectroscopic observation of the subsequent transformation of this species has provided data that demand reappraisal of the general belief that the two subunits of the enzyme function independently.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	41
Volume Number	283
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2008-10-10
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cytochromes Chemistry Nitrite Reductases Nitrites Paracoccus Enzymology Periplasmic Proteins Hydrogen-Ion Concentration Oxidation-Reduction Time Factors Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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