NDLI: Receptor tyrosine kinase Ror2 mediates Wnt5a-induced polarized cell migration by activating c-Jun N-terminal kinase via actin-binding protein filamin A.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nomachi, Akira Minami, Yasuhiro Nishita, Michiru Inaba, Daisuke Enomoto, Masahiro Hamasaki, Mayumi
Description	Author Affiliation: Nomachi A ( Department of Physiology and Cell Biology, Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.)
Abstract	The receptor tyrosine kinase Ror2 has recently been shown to act as an alternative receptor or coreceptor for Wnt5a and to mediate Wnt5a-induced migration of cultured cells. However, little is known about the molecular mechanism underlying this migratory process. Here we show by wound-healing assays that Ror2 plays critical roles in Wnt5a-induced cell migration by regulating formation of lamellipodia and reorientation of microtubule-organizing center (MTOC). Wnt5a stimulation induces activation of the c-Jun N-terminal kinase JNK at the wound edge in a Ror2-dependent manner, and inhibiting JNK activity abrogates Wnt5a-induced lamellipodia formation and MTOC reorientation. Additionally, the association of Ror2 with the actin-binding protein filamin A is required for Wnt5a-induced JNK activation and polarized cell migration. We further show that Wnt5a-induced JNK activation and MTOC reorientation can be suppressed by inhibiting PKCzeta. Taken together, our findings indicate that Wnt5a/Ror2 activates JNK, through a process involving filamin A and PKCzeta, to regulate polarized cell migration.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	41
Volume Number	283
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2008-10-10
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Movement Physiology Cell Polarity Contractile Proteins Metabolism JNK Mitogen-Activated Protein Kinases Microfilament Proteins Receptor Protein-Tyrosine Kinases Wnt Proteins Actins Animals Filamins Mice Microtubule-Organizing Center NIH 3T3 Cells Protein Kinase C Receptor Tyrosine Kinase-like Orphan Receptors Wound Healing Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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