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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Greig, Sarah L. Radjainia, Mazdak Mitra, Alok K. |
| Description | Author Affiliation: Greig SL ( School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.) |
| Abstract | Colicin Ia is a soluble, harpoon-shaped bacteriocin which translocates across the periplasmic space of sensitive Escherichia coli cell by parasitizing an outer membrane receptor and forms voltage-gated ion channels in the inner membrane. This process leads to cell death, which has been thought to be caused by a single colicin Ia molecule. To directly visualize the three-dimensional structure of the channel, we generated two-dimensional crystals of colicin Ia inserted in lipid-bilayer membranes and determined a approximately 17 three-dimensional model by electron crystallography. Supported by velocity sedimentation, chemical cross-linking and single-particle image analysis, the three-dimensional structure is a crown-shaped oligomer enclosing a approximately 35 A-wide extrabilayer vestibule. Our study suggests that lipid insertion instigates a global conformational change in colicin Ia and that more than one molecule participates in the channel architecture with the vestibule, possibly facilitating the known large scale peptide translocation upon channel opening. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 24 |
| Volume Number | 284 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2009-06-12 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacterial Outer Membrane Proteins Chemistry Colicins Escherichia Coli Lipid Bilayers Centrifugation, Density Gradient Isolation & Purification Cross-Linking Reagents Crystallography, X-Ray Ultrastructure Microscopy, Electron Models, Chemical Molecular Conformation Protein Structure, Tertiary Structure-Activity Relationship Sucrose Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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