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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Whitfield, Chris Jones, Michelle L. Hampton, Lauren Nickerson, Nicholas N. Mainprize, Iain L. Naismith, James H. |
| Description | Author Affiliation: Nickerson NN ( Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1); Mainprize IL ( Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1); Hampton L ( Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1); Jones ML ( Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1); Naismith JH ( Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews KY16 9ST, Scotland.); Whitfield C ( Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1); |
| Abstract | The outer membrane (OM) of Gram-negative bacteria is designed to exclude potentially harmful molecules. This property presents a challenge for bacteria that must secrete proteins and large glycoconjugates to grow, divide, and persist. Proteins involved in trafficking such molecules have been identified, but their precise roles are often unresolved due to the difficulty in capturing 'snapshots' during the export pathway. Wza is the prototype for the large family of OM polysaccharide export proteins. In Escherichia coli, Wza is essential for the assembly of a capsule, a protective surface coat composed of long-chain polysaccharides. Wza creates an octameric -helical channel spanning the OM, but the bulk of the protein exists as a large periplasmic structure enclosing an extensive lumen. Residues within the lumen of Wza were targeted for site-specific incorporation of the UV photo-cross-linkable unnatural amino acid p-benzoyl-L-phenylalanine. Using this in vivo photo-cross-linking strategy, we were able to trap polysaccharide translocation intermediates within the lumen of Wza, providing the first unequivocal evidence to our knowledge that nascent capsular polysaccharide chains exit the cell through the Wza portal. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 22 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-06-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacterial Capsules Metabolism Bacterial Outer Membrane Proteins Escherichia Coli Proteins Escherichia Coli Polysaccharides, Bacterial Amino Acid Sequence Chemistry Cross-Linking Reagents Molecular Sequence Data Protein Structure, Quaternary Protein Structure, Tertiary Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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