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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Robin, Gautier Carroll, Bernard J. Forwood, Jade K. Marfori, Mary Kobe, Bostjan Lonhienne, Thierry G. |
| Description | Author Affiliation: Lonhienne TG ( Australian Research Council Centre of Excellence for Integrative Legume Research, School of Chemistry and Molecular Biosciences, University of Queensland, QLD 4072, St. Lucia, Australia. t.lonhienne@uq.edu.au) |
| Abstract | Ran-GTP interacts strongly with importin-beta, and this interaction promotes the release of the importin-alpha-nuclear localization signal cargo from importin-beta. Ran-GDP also interacts with importin-beta, but this interaction is 4 orders of magnitude weaker than the Ran-GTP.importin-beta interaction. Here we use the yeast complement of nuclear import proteins to show that the interaction between Ran-GDP and importin-beta promotes the dissociation of GDP from Ran. The release of GDP from the Ran-GDP-importin-beta complex stabilizes the complex, which cannot be dissociated by importin-alpha. Although Ran has a higher affinity for GDP compared with GTP, Ran in complex with importin-beta has a higher affinity for GTP. This feature is responsible for the generation of Ran-GTP from Ran-GDP by importin-beta. Ran-binding protein-1 (RanBP1) activates this reaction by forming a trimeric complex with Ran-GDP and importin-beta. Importin-alpha inhibits the GDP exchange reaction by sequestering importin-beta, whereas RanBP1 restores the GDP nucleotide exchange by importin-beta by forming a tetrameric complex with importin-beta, Ran, and importin-alpha. The exchange is also inhibited by nuclear-transport factor-2 (NTF2). We suggest a mechanism for nuclear import, additional to the established RCC1 (Ran-guanine exchange factor)-dependent pathway that incorporates these results. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 34 |
| Volume Number | 284 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2009-08-21 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Active Transport, Cell Nucleus Physiology Guanine Nucleotide Exchange Factors Metabolism Beta Karyopherins Ran GTP-Binding Protein Genetics Chromatography, Gel Chromatography, High Pressure Liquid Guanosine Diphosphate Guanosine Triphosphate Nuclear Proteins Protein Binding Alpha Karyopherins Research Support, Non-U.S. Gov't Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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