NDLI: Structural and functional analysis of transmembrane segment IV of the salt tolerance protein Sod2.

Please wait, while we are loading the content...

Year: 2017 Volume: 292

Year: 2016 Volume: 291

Year: 2015 Volume: 290

Year: 2014 Volume: 289

Year: 2013 Volume: 288

Year: 2013 Volume: 288 Issue: 52

Year: 2013 Volume: 288 Issue: 51

Year: 2013 Volume: 288 Issue: 50

Year: 2013 Volume: 288 Issue: 49

Year: 2013 Volume: 288 Issue: 48

Year: 2013 Volume: 288 Issue: 47

Year: 2013 Volume: 288 Issue: 46

Year: 2013 Volume: 288 Issue: 45

Year: 2013 Volume: 288 Issue: 44

Year: 2013 Volume: 288 Issue: 43

Year: 2013 Volume: 288 Issue: 42

Year: 2013 Volume: 288 Issue: 41

Year: 2013 Volume: 288 Issue: 40

Year: 2013 Volume: 288 Issue: 39

Year: 2013 Volume: 288 Issue: 38

Year: 2013 Volume: 288 Issue: 37

Year: 2013 Volume: 288 Issue: 36

Year: 2013 Volume: 288 Issue: 35

Year: 2013 Volume: 288 Issue: 34

Identification and characterization of a periplasmic aminoacyl-phosphatidylglycerol hydrolase responsible for Pseudomonas aeruginosa lipid homeostasis.

Protein-tyrosine phosphatase 1B antagonized signaling by insulin-like growth factor-1 receptor and kinase BRK/PTK6 in ovarian cancer cells.

By ribosome possessed.

c-Abl-dependent molecular circuitry involving Smad5 and phosphatidylinositol 3-kinase regulates bone morphogenetic protein-2-induced osteogenesis.

Insight into pleiotropic drug resistance ATP-binding cassette pump drug transport through mutagenesis of Cdr1p transmembrane domains.

Regulation of Inositol Metabolism Is Fine-tuned by Inositol Pyrophosphates in Saccharomyces cerevisiae

A novel bacterial enzyme with D-glucuronyl C5-epimerase activity.

T-cadherin is essential for adiponectin-mediated revascularization.

The galectin CvGal1 from the eastern oyster (Crassostrea virginica) binds to blood group A oligosaccharides on the hemocyte surface.

Hemocytes and plasma of the eastern oyster (Crassostrea virginica) display a diverse repertoire of sulfated and blood group A-modified N-glycans.

Ankyrin Repeat Domain Protein 2 and Inhibitor of DNA Binding 3 Cooperatively Inhibit Myoblast Differentiation by Physical Interaction

The impact of nitric oxide toxicity on the evolution of the glutathione transferase superfamily: a proposal for an evolutionary driving force.

Transcriptional properties of mammalian elongin A and its role in stress response.

Effects of phosphorylation on the structure and backbone dynamics of the intrinsically disordered connexin43 C-terminal domain.

Structural Basis for the Association of MAP6 Protein with Microtubules and Its Regulation by Calmodulin

Steroid-sensitive gene 1 is a novel cyclic GMP-dependent protein kinase I substrate in vascular smooth muscle cells.

The RNA-binding protein fused in sarcoma (FUS) functions downstream of poly(ADP-ribose) polymerase (PARP) in response to DNA damage.

CRMP2 protein SUMOylation modulates NaV1.7 channel trafficking.

Disassembly of All SNARE Complexes by N-Ethylmaleimide-sensitive Factor (NSF) Is Initiated by a Conserved 1:1 Interaction between α-Soluble NSF Attachment Protein (SNAP) and SNARE $Complex^{*}$

Differential Effects of Mutations on the Transport Properties of the $Na^{+}/H^{+}$ Antiporter NhaA from Escherichia $coli^{*}$

Post-translational Modifications near the Quinone Binding Site of Mammalian Complex $I^{*}$

The transcription factor Atonal homolog 8 regulates Gata4 and Friend of Gata-2 during vertebrate development.

Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase.

Structural and functional analysis of transmembrane segment IV of the salt tolerance protein Sod2.

Peptide modulation of class I major histocompatibility complex protein molecular flexibility and the implications for immune recognition.

Heterodimerization with Its splice variant blocks the ghrelin receptor 1a in a non-signaling conformation: a study with a purified heterodimer assembled into lipid discs.

The size of the unbranched aliphatic chain determines the immunomodulatory potency of short and long chain n-alkanols.

The STRA6 receptor is essential for retinol-binding protein-induced insulin resistance but not for maintaining vitamin A homeostasis in tissues other than the eye.

Ferredoxin Competes with Bacterial Frataxin in Binding to the Desulfurase $IscS^{*}$

Isolating contributions from intersegmental transfer to DNA searching by alkyladenine DNA glycosylase.

Epidermal growth factor (EGF)-induced corneal epithelial wound healing through nuclear factor κB subtype-regulated CCCTC binding factor (CTCF) activation.

Up-regulation of $GABA_{B}$ Receptor Signaling by Constitutive Assembly with the $K^{+}$ Channel Tetramerization Domain-containing Protein 12 $(KCTD12)^{*}$

Identification and structural characterization of a Legionella phosphoinositide phosphatase.

Individual Interactions of the b Subunits within the Stator of the Escherichia coli ATP Synthase

Protein kinase D1-mediated phosphorylations regulate vasodilator-stimulated phosphoprotein (VASP) localization and cell migration.

Endocytosis of Mycobacterium tuberculosis heat shock protein 60 is required to induce interleukin-10 production in macrophages.

Structural and functional evolution of positively selected sites in pine glutathione S-transferase enzyme family.

Disruption of multivesicular body vesicles does not affect major histocompatibility complex (MHC) class II-peptide complex formation and antigen presentation by dendritic cells.

Inhibition of protein kinase C signaling maintains rat embryonic stem cell pluripotency.

DEAF1 Is a Pellino1-interacting Protein Required for Interferon Production by Sendai Virus and Double-stranded $RNA^{*}$

Identification of cargo proteins specific for importin-ß with importin- applying a stable isotope labeling by amino acids in cell culture (SILAC)-based in vitro transport system.

Fine-mapping the contact sites of the Escherichia coli cell division proteins FtsB and FtsL on the FtsQ protein.

Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC).

Proto-oncogene activity of melanoma antigen-A11 (MAGE-A11) regulates retinoblastoma-related p107 and E2F1 proteins.

Foxp3 protein stability is regulated by cyclin-dependent kinase 2.

Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3ß in pancreatic ß-cells.

ADP-ribosylation of translation elongation factor 2 by diphtheria toxin in yeast inhibits translation and cell separation.

MinC protein shortens FtsZ protofilaments by preferentially interacting with GDP-bound subunits.

Interaction of E2F7 transcription factor with E2F1 and C-terminal-binding protein (CtBP) provides a mechanism for E2F7-dependent transcription repression.

Heterogeneous nuclear ribonucleoprotein (HnRNP) K genome-wide binding survey reveals its role in regulating 3'-end RNA processing and transcription termination at the early growth response 1 (EGR1) gene through XRN2 exonuclease.

Hepatitis C Virus NS5A Inhibits Mixed Lineage Kinase 3 to Block $Apoptosis^{*}$

Zinc finger protein 64 promotes Toll-like receptor-triggered proinflammatory and type I interferon production in macrophages by enhancing p65 subunit activation.

SH3-binding Protein 5 Mediates the Neuroprotective Effect of the Secreted Bioactive Peptide Humanin by Inhibiting c-Jun $NH_{2}-terminal$ Kinase

Mediator of ERBB2-driven cell motility (MEMO) promotes extranuclear estrogen receptor signaling involving the growth factor receptors IGF1R and ERBB2.

Lipid segregation and membrane budding induced by the peripheral membrane binding protein annexin A2.

Modulation of GluK2a subunit-containing kainate receptors by 14-3-3 proteins.

Posttranslational Modifications in Type I Collagen from Different Tissues Extracted from Wild Type and Prolyl 3-Hydroxylase 1 Null Mice

A $Ca^{2+}-dependent$ Mechanism of Neuronal Survival Mediated by the Microtubule-associated Protein p600

Tumor cell migration and invasion are enhanced by depletion of Rap1 GTPase-activating protein (Rap1GAP).

Energy Transducing Roles of Antiporter-like Subunits in Escherichia coli NDH-1 with Main Focus on Subunit NuoN (ND2)

Year: 2013 Volume: 288 Issue: 33

Year: 2013 Volume: 288 Issue: 32

Year: 2013 Volume: 288 Issue: 31

Year: 2013 Volume: 288 Issue: 30

Year: 2013 Volume: 288 Issue: 29

Year: 2013 Volume: 288 Issue: 28

Year: 2013 Volume: 288 Issue: 27

Year: 2013 Volume: 288 Issue: 26

Year: 2013 Volume: 288 Issue: 25

Year: 2013 Volume: 288 Issue: 24

Year: 2013 Volume: 288 Issue: 23

Year: 2013 Volume: 288 Issue: 22

Year: 2013 Volume: 288 Issue: 21

Year: 2013 Volume: 288 Issue: 20

Year: 2013 Volume: 288 Issue: 19

Year: 2013 Volume: 288 Issue: 18

Year: 2013 Volume: 288 Issue: 17

Year: 2013 Volume: 288 Issue: 16

Year: 2013 Volume: 288 Issue: 15

Year: 2013 Volume: 288 Issue: 14

Year: 2013 Volume: 288 Issue: 13

Year: 2013 Volume: 288 Issue: 12

Year: 2013 Volume: 288 Issue: 11

Year: 2013 Volume: 288 Issue: 10

Year: 2013 Volume: 288 Issue: 9

Year: 2013 Volume: 288 Issue: 8

Year: 2013 Volume: 288 Issue: 7

Year: 2013 Volume: 288 Issue: 6

Year: 2013 Volume: 288 Issue: 5

Year: 2013 Volume: 288 Issue: 4

Year: 2013 Volume: 288 Issue: 3

Year: 2013 Volume: 288 Issue: 2

Year: 2013 Volume: 288 Issue: 1

Year: 2012 Volume: 287

Year: 2011 Volume: 286

Year: 2010 Volume: 285

Year: 2009 Volume: 284

Year: 2008 Volume: 283

Year: 2007 Volume: 282

Year: 2006 Volume: 281

Year: 2005 Volume: 280

Year: 2004 Volume: 279

Year: 2003 Volume: 278

Year: 2002 Volume: 277

Year: 2001 Volume: 276

Year: 2000 Volume: 275

Year: 1999 Volume: 274

Year: 1998 Volume: 273

Year: 1997 Volume: 272

Year: 1996 Volume: 271

Year: 1995 Volume: 270

Year: 1994 Volume: 269

Year: 1993 Volume: 268

Year: 1992 Volume: 267

Year: 1991 Volume: 266

Year: 1990 Volume: 265

Year: 1989 Volume: 264

Year: 1988 Volume: 263

Year: 1987 Volume: 262

Year: 1986 Volume: 261

Year: 1984 Volume: 259

Year: 1982 Volume: 257

Year: 1981 Volume: 256

Year: 1975 Volume: 250

Structural and functional analysis of transmembrane segment IV of the salt tolerance protein Sod2.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kemp, Grant Young, Howard Alves, Claudia Fliegel, Larry Ullah, Asad Lee, Brian Sykes, Brian D.
Description	Author Affiliation: Ullah A ( Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.)
Abstract	Sod2 is the plasma membrane Na(+)/H(+) exchanger of the fission yeast Schizosaccharomyces pombe. It provides salt tolerance by removing excess intracellular sodium (or lithium) in exchange for protons. We examined the role of amino acid residues of transmembrane segment IV (TM IV) ((126)FPQINFLGSLLIAGCITSTDPVLSALI(152)) in activity by using alanine scanning mutagenesis and examining salt tolerance in sod2-deficient S. pombe. Two amino acids were critical for function. Mutations T144A and V147A resulted in defective proteins that did not confer salt tolerance when reintroduced into S. pombe. Sod2 protein with other alanine mutations in TM IV had little or no effect. T144D and T144K mutant proteins were inactive; however, a T144S protein was functional and provided lithium, but not sodium, tolerance and transport. Analysis of sensitivity to trypsin indicated that the mutations caused a conformational change in the Sod2 protein. We expressed and purified TM IV (amino acids 125-154). NMR analysis yielded a model with two helical regions (amino acids 128-142 and 147-154) separated by an unwound region (amino acids 143-146). Molecular modeling of the entire Sod2 protein suggested that TM IV has a structure similar to that deduced by NMR analysis and an overall structure similar to that of Escherichia coli NhaA. TM IV of Sod2 has similarities to TM V of the Zygosaccharomyces rouxii Na(+)/H(+) exchanger and TM VI of isoform 1 of mammalian Na(+)/H(+) exchanger. TM IV of Sod2 is critical to transport and may be involved in cation binding or conformational changes of the protein.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	34
Volume Number	288
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2013-08-23
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Schizosaccharomyces Pombe Proteins Metabolism Schizosaccharomyces Sodium-Hydrogen Antiporter Amino Acid Substitution Escherichia Coli Chemistry Genetics Escherichia Coli Proteins Fungal Proteins Mutation, Missense Nuclear Magnetic Resonance, Biomolecular Protein Structure, Secondary Protein Structure, Tertiary Structural Homology, Protein Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in