NDLI: Insulin Protects Pancreatic Acinar Cells from Palmitoleic Acid-induced Cellular $Injury^{*}$

Please wait, while we are loading the content...

Year: 2017 Volume: 292

Year: 2016 Volume: 291

Year: 2015 Volume: 290

Year: 2014 Volume: 289

Year: 2014 Volume: 289 Issue: 52

Year: 2014 Volume: 289 Issue: 51

Year: 2014 Volume: 289 Issue: 50

Year: 2014 Volume: 289 Issue: 49

Year: 2014 Volume: 289 Issue: 48

Year: 2014 Volume: 289 Issue: 47

Year: 2014 Volume: 289 Issue: 46

Year: 2014 Volume: 289 Issue: 45

Year: 2014 Volume: 289 Issue: 44

Year: 2014 Volume: 289 Issue: 43

Year: 2014 Volume: 289 Issue: 42

Year: 2014 Volume: 289 Issue: 41

Year: 2014 Volume: 289 Issue: 40

Year: 2014 Volume: 289 Issue: 39

Year: 2014 Volume: 289 Issue: 38

Year: 2014 Volume: 289 Issue: 37

Year: 2014 Volume: 289 Issue: 36

Year: 2014 Volume: 289 Issue: 35

Year: 2014 Volume: 289 Issue: 34

Molecular characterization of quinate and shikimate metabolism in Populus trichocarpa.

Solution NMR structure and functional analysis of the integral membrane protein YgaP from Escherichia coli.

Acidification asymmetrically affects voltage-dependent anion channel implicating the involvement of salt bridges.

Leucine-rich repeat-containing G-protein-coupled Receptor 5 marks short-term hematopoietic stem and progenitor cells during mouse embryonic development.

Inhibition of Plasma Kallikrein by a Highly Specific Active Site Blocking Antibody

Effects of a Disrupted Blood-Brain Barrier on Cholesterol Homeostasis in the $Brain^{*}$

The structure of the cytomegalovirus-encoded m04 glycoprotein, a prototypical member of the m02 family of immunoevasins.

Cancer-associated isocitrate dehydrogenase 1 (IDH1) R132H mutation and d-2-hydroxyglutarate stimulate glutamine metabolism under hypoxia.

Umbilical cord mesenchymal stem cells suppress host rejection: the role of the glycocalyx.

Direct evidence of an elongation factor-Tu/Ts·GTP·Aminoacyl-tRNA quaternary complex.

Biochemical and structural studies of 6-carboxy-5,6,7,8-tetrahydropterin synthase reveal the molecular basis of catalytic promiscuity within the tunnel-fold superfamily.

Structure of the type IX group B Streptococcus capsular polysaccharide and its evolutionary relationship with types V and VII.

Molecular and immunological characterization of the first allergenic lipocalin in hamster: the major allergen from Siberian hamster (Phodopus sungorus).

Functional Analysis of Dishevelled-3 Phosphorylation Identifies Distinct Mechanisms Driven by Casein Kinase 1ϵ and $Frizzled5^{*}$

Functional effects of a pathogenic mutation in Cereblon (CRBN) on the regulation of protein synthesis via the AMPK-mTOR cascade.

The G protein-coupled receptor P2Y14 influences insulin release and smooth muscle function in mice.

NMR-based structural analysis of the complete rough-type lipopolysaccharide isolated from Capnocytophaga canimorsus.

Biophysical optimization of a therapeutic protein by nonstandard mutagenesis: studies of an iodo-insulin derivative.

Insulin Protects Pancreatic Acinar Cells from Palmitoleic Acid-induced Cellular $Injury^{*}$

Defining the structure and receptor binding domain of the leaderless bacteriocin LsbB.

Structural Insights into the Activation of the RhoA GTPase by the Lymphoid Blast Crisis (Lbc) $Oncoprotein^{*}$

Hepatic differentiated embryo-chondrocyte-expressed gene 1 (Dec1) inhibits sterol regulatory element-binding protein-1c (Srebp-1c) expression and alleviates fatty liver phenotype.

DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes.

MicroRNA-130a is up-regulated in mouse liver by iron deficiency and targets the bone morphogenetic protein (BMP) receptor ALK2 to attenuate BMP signaling and hepcidin transcription.

The Combined Structural and Kinetic Characterization of a Bacterial Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 Establishes NMO Class I and II

Deciphering a molecular mechanism of neonatal diabetes mellitus by the chemical synthesis of a protein diastereomer, [D-AlaB8]human proinsulin.

Crystal structure and site-directed mutational analysis reveals key residues involved in Escherichia coli ZapA function.

Site-directed fluorescence labeling reveals a revised N-terminal membrane topology and functional periplasmic residues in the Escherichia coli cell division protein FtsK.

Phosphatase 1 nuclear targeting subunit is an essential regulator of M-phase entry, maintenance, and exit.

Susceptibility to HLA-DM Protein Is Determined by a Dynamic Conformation of Major Histocompatibility Complex Class II Molecule Bound with Peptide

Interferon Î³-inducible protein (IFI) 16 transcriptionally regulates type i interferons and other interferon-stimulated genes and controls the interferon response to both DNA and RNA viruses.

Structure of pneumococcal peptidoglycan hydrolase LytB reveals insights into the bacterial cell wall remodeling and pathogenesis.

Retinoblastoma protein and MyoD function together to effect the repression of Fra-1 and in turn cyclin D1 during terminal cell cycle arrest associated with myogenesis.

A Conserved Mitochondrial ATP-binding Cassette Transporter Exports Glutathione Polysulfide for Cytosolic Metal Cofactor $Assembly^{*♦}$

Apoptosis-linked gene-2 (ALG-2)/Sec31 interactions regulate endoplasmic reticulum (ER)-to-Golgi transport: a potential effector pathway for luminal calcium.

Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly.

The conformational changes induced by ubiquinone binding in the Na+-pumping NADH:ubiquinone oxidoreductase (Na+-NQR) are kinetically controlled by conserved glycines 140 and 141 of the NqrB subunit.

Magnesium Modulates Actin Binding and ADP Release in Myosin Motors

Molecular mechanisms of bitopic ligand engagement with the M1 muscarinic acetylcholine receptor.

The p38ß mitogen-activated protein kinase possesses an intrinsic autophosphorylation activity, generated by a short region composed of the -G helix and MAPK insert.

Open Chromatin Profiling in Mice Livers Reveals Unique Chromatin Variations Induced by High Fat Diet

Vesicle-associated Membrane Protein 2 (VAMP2) but Not VAMP3 Mediates cAMP-stimulated Trafficking of the Renal $Na^{+}-K^{+}-2Cl^{−}$ Co-transporter NKCC2 in Thick Ascending $Limbs^{*}$

Concerted All-or-none Subunit Interactions Mediate Slow Deactivation of Human ether-à-go-go-related Gene $K^{+}$ Channels

A WXW Motif Is Required for the Anticancer Activity of the $TAT-RasGAP_{317–326}$ Peptide

The PsbP domain protein 1 functions in the assembly of lumenal domains in photosystem I.

PrkC-mediated Phosphorylation of Overexpressed YvcK Protein Regulates PBP1 Protein Localization in Bacillus subtilis mreB Mutant Cells

The molecular mechanism of eukaryotic elongation factor 2 kinase activation.

Egr1 protein acts downstream of estrogen-leukemia inhibitory factor (LIF)-STAT3 pathway and plays a role during implantation through targeting Wnt4.

Thyroid hormone receptor interacting protein 13 (TRIP13) AAA-ATPase is a novel mitotic checkpoint-silencing protein.

Interferon- acts on the S/G2/M phases to induce apoptosis in the G1 phase of an IFNAR2-expressing hepatocellular carcinoma cell line.

Plasticity and evolution of (+)-3-carene synthase and (-)-sabinene synthase functions of a sitka spruce monoterpene synthase gene family associated with weevil resistance.

Differential Regulation of Endosomal GPCR/β-Arrestin Complexes and Trafficking by MAPK

Paradoxical role of DNA methylation in activation of FoxA2 gene expression during endoderm development.

Prolactin (PRL)-stimulated ubiquitination of ZnT2 mediates a transient increase in zinc secretion followed by ZnT2 degradation in mammary epithelial cells.

Bcl-xL protein protects from C/EBP homologous protein (CHOP)-dependent apoptosis during plasma cell differentiation.

Stearoyl-CoA desaturase 1 activity is required for autophagosome formation.

PTPN14 forms a complex with Kibra and LATS1 proteins and negatively regulates the YAP oncogenic function.

Regulation of protein phosphatase 1I by Cdc25C-associated kinase 1 (C-TAK1) and PFTAIRE protein kinase.

Assembly mechanism of Trypanosoma brucei BILBO1, a multidomain cytoskeletal protein.

Identification of the docking site between a type III secretion system ATPase and a chaperone for effector cargo.

Retraction: Structural studies on molecular interactions between camel peptidoglycan recognition protein, CPGRP-S, and peptidoglycan moieties N-acetylglucosamine and N-acetylmuramic acid.

Year: 2014 Volume: 289 Issue: 33

Year: 2014 Volume: 289 Issue: 32

Year: 2014 Volume: 289 Issue: 31

Year: 2014 Volume: 289 Issue: 30

Year: 2014 Volume: 289 Issue: 29

Year: 2014 Volume: 289 Issue: 28

Year: 2014 Volume: 289 Issue: 27

Year: 2014 Volume: 289 Issue: 26

Year: 2014 Volume: 289 Issue: 25

Year: 2014 Volume: 289 Issue: 24

Year: 2014 Volume: 289 Issue: 23

Year: 2014 Volume: 289 Issue: 22

Year: 2014 Volume: 289 Issue: 21

Year: 2014 Volume: 289 Issue: 20

Year: 2014 Volume: 289 Issue: 19

Year: 2014 Volume: 289 Issue: 18

Year: 2014 Volume: 289 Issue: 17

Year: 2014 Volume: 289 Issue: 16

Year: 2014 Volume: 289 Issue: 15

Year: 2014 Volume: 289 Issue: 14

Year: 2014 Volume: 289 Issue: 13

Year: 2014 Volume: 289 Issue: 12

Year: 2014 Volume: 289 Issue: 11

Year: 2014 Volume: 289 Issue: 10

Year: 2014 Volume: 289 Issue: 9

Year: 2014 Volume: 289 Issue: 8

Year: 2014 Volume: 289 Issue: 7

Year: 2014 Volume: 289 Issue: 6

Year: 2014 Volume: 289 Issue: 5

Year: 2014 Volume: 289 Issue: 4

Year: 2014 Volume: 289 Issue: 3

Year: 2014 Volume: 289 Issue: 2

Year: 2014 Volume: 289 Issue: 1

Year: 2013 Volume: 288

Year: 2012 Volume: 287

Year: 2011 Volume: 286

Year: 2010 Volume: 285

Year: 2009 Volume: 284

Year: 2008 Volume: 283

Year: 2007 Volume: 282

Year: 2006 Volume: 281

Year: 2005 Volume: 280

Year: 2004 Volume: 279

Year: 2003 Volume: 278

Year: 2002 Volume: 277

Year: 2001 Volume: 276

Year: 2000 Volume: 275

Year: 1999 Volume: 274

Year: 1998 Volume: 273

Year: 1997 Volume: 272

Year: 1996 Volume: 271

Year: 1995 Volume: 270

Year: 1994 Volume: 269

Year: 1993 Volume: 268

Year: 1992 Volume: 267

Year: 1991 Volume: 266

Year: 1990 Volume: 265

Year: 1989 Volume: 264

Year: 1988 Volume: 263

Year: 1987 Volume: 262

Year: 1986 Volume: 261

Year: 1984 Volume: 259

Year: 1982 Volume: 257

Year: 1981 Volume: 256

Year: 1975 Volume: 250

Insulin Protects Pancreatic Acinar Cells from Palmitoleic Acid-induced Cellular $Injury^{*}$

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wong, James Patel, Waseema Bruce, Jason I. E. Whitehouse, John Alves-simoes, Marta Mankad, Parini Samad, Aysha James, Andrew Siriwardena, Ajith K.
Description	Author Affiliation: Samad A ( From the Faculty of Life Sciences, The University of Manchester, M13 9NT Manchester and.); James A ( From the Faculty of Life Sciences, The University of Manchester, M13 9NT Manchester and.); Wong J ( From the Faculty of Life Sciences, The University of Manchester, M13 9NT Manchester and.); Mankad P ( From the Faculty of Life Sciences, The University of Manchester, M13 9NT Manchester and.); Whitehouse J ( From the Faculty of Life Sciences, The University of Manchester, M13 9NT Manchester and.); Patel W ( From the Faculty of Life Sciences, The University of Manchester, M13 9NT Manchester and.); Alves-Simoes M ( From the Faculty of Life Sciences, The University of Manchester, M13 9NT Manchester and.); Siriwardena AK ( the Hepatobiliary Surgery Unit, Manchester Royal Infirmary, M13 9WL Manchester, United Kingdom.); Bruce JI ( From the Faculty of Life Sciences, The University of Manchester, M13 9NT Manchester and jason.bruce@manchester.ac.uk.)
Abstract	Acute pancreatitis is a serious and sometimes fatal inflammatory disease where the pancreas digests itself. The non-oxidative ethanol metabolites palmitoleic acid (POA) and POA-ethylester (POAEE) are reported to induce pancreatitis caused by impaired mitochondrial metabolism, cytosolic $Ca^{2+}$ $([Ca^{2+}]_{i})$ overload and necrosis of pancreatic acinar cells. Metabolism and $[Ca^{2+}]_{i}$ are linked critically by the ATP-driven plasma membrane $Ca^{2+}-ATPase$ (PMCA) important for maintaining low resting $[Ca^{2+}]_{i}.$ The aim of the current study was to test the protective effects of insulin on cellular injury induced by the pancreatitis-inducing agents, ethanol, POA, and POAEE. Rat pancreatic acinar cells were isolated by collagenase digestion and $[Ca^{2+}]_{i}$ was measured by fura-2 imaging. An in situ $[Ca^{2+}]_{i}$ clearance assay was used to assess PMCA activity. Magnesium green (MgGreen) and a luciferase-based ATP kit were used to assess cellular ATP depletion. Ethanol (100 mm) and POAEE (100 μm) induced a small but irreversible $Ca^{2+}$ overload response but had no significant effect on PMCA activity. POA (50–100 μm) induced a robust $Ca^{2+}$ overload, ATP depletion, inhibited PMCA activity, and consequently induced necrosis. Insulin pretreatment (100 nm for 30 min) prevented the POA-induced $Ca^{2+}$ overload, ATP depletion, inhibition of the PMCA, and necrosis. Moreover, the insulin-mediated protection of the POA-induced $Ca^{2+}$ overload was partially prevented by the phosphoinositide-3-kinase (PI3K) inhibitor, LY294002. These data provide the first evidence that insulin directly protects pancreatic acinar cell injury induced by bona fide pancreatitis-inducing agents, such as POA. This may have important therapeutic implications for the treatment of pancreatitis.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	34
Volume Number	289
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2014-08-22
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Fatty Acids, Monounsaturated Pharmacology Insulin Physiology Pancreas Drug Effects Adenosine Triphosphate Metabolism Animals Calcium Cell Death Chromones Ethanol Administration & Dosage Fatty Acids Fluorescence Morpholines Cytology Plasma Membrane Calcium-Transporting ATPases Rats, Sprague-Dawley Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in