NDLI: Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency.

Please wait, while we are loading the content...

Year: 2017 Volume: 292

Year: 2016 Volume: 291

Year: 2015 Volume: 290

Year: 2014 Volume: 289

Year: 2014 Volume: 289 Issue: 52

Year: 2014 Volume: 289 Issue: 51

Year: 2014 Volume: 289 Issue: 50

Year: 2014 Volume: 289 Issue: 49

Year: 2014 Volume: 289 Issue: 48

Year: 2014 Volume: 289 Issue: 47

Year: 2014 Volume: 289 Issue: 46

Year: 2014 Volume: 289 Issue: 45

Year: 2014 Volume: 289 Issue: 44

Year: 2014 Volume: 289 Issue: 43

Protein dynamics control the progression and efficiency of the catalytic reaction cycle of the Escherichia coli DNA-repair enzyme AlkB.

Systematic analysis of bacterial effector-postsynaptic density 95/disc large/zonula occludens-1 (PDZ) domain interactions demonstrates Shigella OspE protein promotes protein kinase C activation via PDLIM proteins.

Gene targeting study reveals unexpected expression of brain-expressed X-linked 2 in endocrine and tissue stem/progenitor cells in mice.

Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase.

Cardiomyocyte-specific loss of diacylglycerol acyltransferase 1 (DGAT1) reproduces the abnormalities in lipids found in severe heart failure.

Targeting of splice variants of human cytochrome P450 2C8 (CYP2C8) to mitochondria and their role in arachidonic acid metabolism and respiratory dysfunction.

A fusion intermediate gp41 immunogen elicits neutralizing antibodies to HIV-1.

PDK4 protein promotes tumorigenesis through activation of cAMP-response element-binding protein (CREB)-Ras homolog enriched in brain (RHEB)-mTORC1 signaling cascade.

The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis.

Serine racemase regulated by binding to stargazin and PSD-95: potential N-methyl-D-aspartate- -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (NMDA-AMPA) glutamate neurotransmission cross-talk.

Fibroblast growth factor 21 is regulated by the IRE1 -XBP1 branch of the unfolded protein response and counteracts endoplasmic reticulum stress-induced hepatic steatosis.

Crystal structure of the ectoine hydroxylase, a snapshot of the active site.

Computational and biochemical docking of the irreversible cocaine analog RTI 82 directly demonstrates ligand positioning in the dopamine transporter central substrate-binding site.

MicroRNA-539 is up-regulated in failing heart, and suppresses O-GlcNAcase expression.

Selective impairment of a subset of Ran-GTP-binding domains of ran-binding protein 2 (Ranbp2) suffices to recapitulate the degeneration of the retinal pigment epithelium (RPE) triggered by Ranbp2 ablation.

Essential role of the zinc finger transcription factor Casz1 for mammalian cardiac morphogenesis and development.

Regulation of lipogenic gene expression by lysine-specific histone demethylase-1 (LSD1).

Evolutionary conservation in biogenesis of ß-barrel proteins allows mitochondria to assemble a functional bacterial trimeric autotransporter protein.

Structural Insights into the Mechanism of Negative Regulation of Single-box High Mobility Group Proteins by the Acidic Tail $Domain^{*}$

Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force $Microscopy^{*}$

Membrane-induced allosteric control of phospholipase C-ß isozymes.

Carbon Monoxide-releasing Molecule-3 (CORM-3; $Ru(CO)_{3}Cl(Glycinate))$ as a Tool to Study the Concerted Effects of Carbon Monoxide and Nitric Oxide on Bacterial Flavohemoglobin Hmp: APPLICATIONS AND PITFALLS

The catabolite control protein E (CcpE) affects virulence determinant production and pathogenesis of Staphylococcus aureus.

Structural basis for the binding specificity of human Recepteur d'Origine Nantais (RON) receptor tyrosine kinase to macrophage-stimulating protein.

Selectivity and evolutionary divergence of metabotropic glutamate receptors for endogenous ligands and G proteins coupled to phospholipase C or TRP channels.

DNA sequences proximal to human mitochondrial DNA deletion breakpoints prevalent in human disease form G-quadruplexes, a class of DNA structures inefficiently unwound by the mitochondrial replicative Twinkle helicase.

Protein/protein interactions in the mammalian heme degradation pathway: heme oxygenase-2, cytochrome P450 reductase, and biliverdin reductase.

The LQLP Calcineurin Docking Site Is a Major Determinant of the Calcium-dependent Activation of Human TRESK Background $K^{+}$ Channel

Asp-52 in combination with Asp-398 plays a critical role in ATP hydrolysis of chaperonin GroEL.

The LIMP-2/SCARB2 binding motif on acid ß-glucosidase: basic and applied implications for Gaucher disease and associated neurodegenerative diseases.

Thioredoxin-dependent redox regulation of chloroplastic phosphoglycerate kinase from Chlamydomonas reinhardtii.

Osmotic stress-induced phosphorylation of H2AX by polo-like kinase 3 affects cell cycle progression in human corneal epithelial cells.

Antimicrobial Effects of Helix D-derived Peptides of Human Antithrombin III

Using Markov state models to develop a mechanistic understanding of protein kinase A regulatory subunit RI activation in response to cAMP binding.

Xenobiotic pregnane X receptor (PXR) regulates innate immunity via activation of NLRP3 inflammasome in vascular endothelial cells.

Transglutaminase II/MicroRNA-218/-181a Loop Regulates Positive Feedback Relationship between Allergic Inflammation and Tumor Metastasis

C-type lectin receptor dectin-3 mediates trehalose 6,6'-dimycolate (TDM)-induced Mincle expression through CARD9/Bcl10/MALT1-dependent nuclear factor (NF)-κB activation.

Kinase-associated endopeptidase 1 (Kae1) participates in an atypical ribosome-associated complex in the apicoplast of Plasmodium falciparum.

Loss of TRB3 alters dynamics of MLK3-JNK signaling and inhibits cytokine-activated pancreatic beta cell death.

PHD Finger Protein 2 (PHF2) Represses Ribosomal RNA Gene Transcription by Antagonizing PHF Finger Protein 8 (PHF8) and Recruiting Methyltransferase SUV39H1

Human 2-oxoglutarate dehydrogenase complex E1 component forms a thiamin-derived radical by aerobic oxidation of the enamine intermediate.

Mechanisms of Neutralization of a Human Anti-α-toxin $Antibody^{*}$

Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis.

Trefoil Factor Family Domains Represent Highly Efficient Conformational Determinants for N-Linked N,N′-di-N-acetyllactosediamine (LacdiNAc) Synthesis

Molecular insight into the role of the N-terminal extension in the maturation, substrate recognition, and catalysis of a bacterial alginate lyase from polysaccharide lyase family 18.

Novel binding motif and new flexibility revealed by structural analyses of a pyruvate dehydrogenase-dihydrolipoyl acetyltransferase subcomplex from the Escherichia coli pyruvate dehydrogenase multienzyme complex.

Duplication of genes in an ATP-binding cassette transport system increases dynamic range while maintaining ligand specificity.

In vivo inhibition of RIPK2 kinase alleviates inflammatory disease.

Inhibiting monoacylglycerol acyltransferase 1 ameliorates hepatic metabolic abnormalities but not inflammation and injury in mice.

Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency.

A signal transducer and activator of transcription 3·Nuclear Factor κB (Stat3·NFκB) complex is necessary for the expression of fascin in metastatic breast cancer cells in response to interleukin (IL)-6 and tumor necrosis factor (TNF)- .

RNA virus population diversity, an optimum for maximal fitness and virulence.

Short mitochondrial ARF triggers Parkin/PINK1-dependent mitophagy.

A C-terminal membrane anchor affects the interactions of prion proteins with lipid membranes.

Collapsin response mediator protein 4 regulates growth cone dynamics through the actin and microtubule cytoskeleton.

Year: 2014 Volume: 289 Issue: 42

Year: 2014 Volume: 289 Issue: 41

Year: 2014 Volume: 289 Issue: 40

Year: 2014 Volume: 289 Issue: 39

Year: 2014 Volume: 289 Issue: 38

Year: 2014 Volume: 289 Issue: 37

Year: 2014 Volume: 289 Issue: 36

Year: 2014 Volume: 289 Issue: 35

Year: 2014 Volume: 289 Issue: 34

Year: 2014 Volume: 289 Issue: 33

Year: 2014 Volume: 289 Issue: 32

Year: 2014 Volume: 289 Issue: 31

Year: 2014 Volume: 289 Issue: 30

Year: 2014 Volume: 289 Issue: 29

Year: 2014 Volume: 289 Issue: 28

Year: 2014 Volume: 289 Issue: 27

Year: 2014 Volume: 289 Issue: 26

Year: 2014 Volume: 289 Issue: 25

Year: 2014 Volume: 289 Issue: 24

Year: 2014 Volume: 289 Issue: 23

Year: 2014 Volume: 289 Issue: 22

Year: 2014 Volume: 289 Issue: 21

Year: 2014 Volume: 289 Issue: 20

Year: 2014 Volume: 289 Issue: 19

Year: 2014 Volume: 289 Issue: 18

Year: 2014 Volume: 289 Issue: 17

Year: 2014 Volume: 289 Issue: 16

Year: 2014 Volume: 289 Issue: 15

Year: 2014 Volume: 289 Issue: 14

Year: 2014 Volume: 289 Issue: 13

Year: 2014 Volume: 289 Issue: 12

Year: 2014 Volume: 289 Issue: 11

Year: 2014 Volume: 289 Issue: 10

Year: 2014 Volume: 289 Issue: 9

Year: 2014 Volume: 289 Issue: 8

Year: 2014 Volume: 289 Issue: 7

Year: 2014 Volume: 289 Issue: 6

Year: 2014 Volume: 289 Issue: 5

Year: 2014 Volume: 289 Issue: 4

Year: 2014 Volume: 289 Issue: 3

Year: 2014 Volume: 289 Issue: 2

Year: 2014 Volume: 289 Issue: 1

Year: 2013 Volume: 288

Year: 2012 Volume: 287

Year: 2011 Volume: 286

Year: 2010 Volume: 285

Year: 2009 Volume: 284

Year: 2008 Volume: 283

Year: 2007 Volume: 282

Year: 2006 Volume: 281

Year: 2005 Volume: 280

Year: 2004 Volume: 279

Year: 2003 Volume: 278

Year: 2002 Volume: 277

Year: 2001 Volume: 276

Year: 2000 Volume: 275

Year: 1999 Volume: 274

Year: 1998 Volume: 273

Year: 1997 Volume: 272

Year: 1996 Volume: 271

Year: 1995 Volume: 270

Year: 1994 Volume: 269

Year: 1993 Volume: 268

Year: 1992 Volume: 267

Year: 1991 Volume: 266

Year: 1990 Volume: 265

Year: 1989 Volume: 264

Year: 1988 Volume: 263

Year: 1987 Volume: 262

Year: 1986 Volume: 261

Year: 1984 Volume: 259

Year: 1982 Volume: 257

Year: 1981 Volume: 256

Year: 1975 Volume: 250

Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kamynina, Elena Lamarre, Simon G. Agunloye, Olufunmilayo C. Brosnan, John T. Stover, Patrick J. Brosnan, Margaret E. Field, Martha S. Liebenthal, Rebecca P.
Description	Author Affiliation: Field MS ( From the Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 and.); Kamynina E ( From the Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 and.); Agunloye OC ( From the Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 and.); Liebenthal RP ( From the Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 and.); Lamarre SG ( Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland A1B 3X9, Canada.); Brosnan ME ( Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland A1B 3X9, Canada.); Brosnan JT ( Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland A1B 3X9, Canada.); Stover PJ ( From the Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853 and pjs13@cornell.edu.)
Abstract	Folate-mediated one-carbon metabolism is a metabolic network of interconnected pathways that is required for the de novo synthesis of three of the four DNA bases and the remethylation of homocysteine to methionine. Previous studies have indicated that the thymidylate synthesis and homocysteine remethylation pathways compete for a limiting pool of methylenetetrahydrofolate cofactors and that thymidylate biosynthesis is preserved in folate deficiency at the expense of homocysteine remethylation, but the mechanisms are unknown. Recently, it was shown that thymidylate synthesis occurs in the nucleus, whereas homocysteine remethylation occurs in the cytosol. In this study we demonstrate that methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), an enzyme that generates methylenetetrahydrofolate from formate, ATP, and NADPH, functions in the nucleus to support de novo thymidylate biosynthesis. MTHFD1 translocates to the nucleus in S-phase MCF-7 and HeLa cells. During folate deficiency mouse liver MTHFD1 levels are enriched in the nucleus >2-fold at the expense of levels in the cytosol. Furthermore, nuclear folate levels are resistant to folate depletion when total cellular folate levels are reduced by >50% in mouse liver. The enrichment of folate cofactors and MTHFD1 protein in the nucleus during folate deficiency in mouse liver and human cell lines accounts for previous metabolic studies that indicated 5,10-methylenetetrahydrofolate is preferentially directed toward de novo thymidylate biosynthesis at the expense of homocysteine remethylation during folate deficiency.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	43
Volume Number	289
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2014-10-24
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Nucleus Metabolism Coenzymes Folic Acid Deficiency Enzymology Folic Acid Methylenetetrahydrofolate Dehydrogenase (NADP) Thymidine Monophosphate Biosynthesis Animals Cell Cycle Checkpoints Cell Line DNA Diet Pathology Formates Blood Gene Knockdown Techniques Glycine Hydroxymethyltransferase Liver Methionine Mice Mice, Inbred C57BL Protein Transport Purines S Phase Uracil Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in