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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, Qiong-yan Zhou, Meng-ting Jiang, Li-jun Liu, Chun Xia, Qing-you Zhao, Xiao-ming Mita, Kazuei Cheng, Ting-cai |
| Description | Author Affiliation: Zhao XM ( From the State Key Laboratory of Silkworm Genome Biology and Key Sericultural Laboratory of the Ministry of Agriculture, College of Bio-Technology, Southwest University, Chongqing 400716 and the Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.); Liu C ( From the State Key Laboratory of Silkworm Genome Biology and Key Sericultural Laboratory of the Ministry of Agriculture, College of Bio-Technology, Southwest University, Chongqing 400716 and mlliuchun@163.com.); Jiang LJ ( From the State Key Laboratory of Silkworm Genome Biology and Key Sericultural Laboratory of the Ministry of Agriculture, College of Bio-Technology, Southwest University, Chongqing 400716 and.); Li QY ( From the State Key Laboratory of Silkworm Genome Biology and Key Sericultural Laboratory of the Ministry of Agriculture, College of Bio-Technology, Southwest University, Chongqing 400716 and.); Zhou MT ( From the State Key Laboratory of Silkworm Genome Biology and Key Sericultural Laboratory of the Ministry of Agriculture, College of Bio-Technology, Southwest University, Chongqing 400716 and.); Cheng TC ( From the State Key Laboratory of Silkworm Genome Biology and Key Sericultural Laboratory of the Ministry of Agriculture, College of Bio-Technology, Southwest University, Chongqing 400716 and.); Mita K ( From the State Key Laboratory of Silkworm Genome Biology and.); Xia QY ( From the State Key Laboratory of Silkworm Genome Biology and Key Sericultural Laboratory of the Ministry of Agriculture, College of Bio-Technology, Southwest University, Chongqing 400716 and xiaqy@swu.edu.cn.) |
| Abstract | The genes responsible for silk biosynthesis are switched on and off at particular times in the silk glands of Bombyx mori. This switch appears to be under the control of endogenous and exogenous hormones. However, the molecular mechanisms by which silk protein synthesis is regulated by the juvenile hormone (JH) are largely unknown. Here, we report a basic helix-loop-helix transcription factor, Bmdimm, its silk gland-specific expression, and its direct involvement in the regulation of fibroin H-chain (fib-H) by binding to an E-box (CAAATG) element of the fib-H gene promoter. Far-Western blots, enzyme-linked immunosorbent assays, and co-immunoprecipitation assays revealed that Bmdimm protein interacted with another basic helix-loop-helix transcription factor, Bmsage. Immunostaining revealed that Bmdimm and Bmsage proteins are co-localized in nuclei. Bmdimm expression was induced in larval silk glands in vivo, in silk glands cultured in vitro, and in B. mori cell lines after treatment with a JH analog. The JH effect on Bmdimm was mediated by the JH-Met-Kr-h1 signaling pathway, and Bmdimm expression did not respond to JH by RNA interference with double-stranded BmKr-h1 RNA. These data suggest that the JH regulatory pathway, the transcription factor Bmdimm, and the targeted fib-H gene contribute to the synthesis of fibroin H-chain protein in B. mori. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 2 |
| Volume Number | 290 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2015-01-09 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Basic Helix-Loop-Helix Transcription Factors Genetics Fibroins Insect Proteins Juvenile Hormones Silk Biosynthesis Animals Bombyx Metabolism Larva Promoter Regions, Genetic Sericins Research Support, Non-U.S. Gov't Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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