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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lai, Tsz-pui Sun, Hongzhe Li, Hongyan Yang, Xinming |
| Description | Author Affiliation: Yang X ( From the Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.); Li H ( From the Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.); Lai TP ( From the Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.); Sun H ( From the Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China hsun@hku.hk.) |
| Abstract | The pathogenicity of Helicobacter pylori relies heavily on urease, which converts urea to ammonia to neutralize the stomach acid. Incorporation of Ni(2+) into the active site of urease requires a battery of chaperones. Both metallochaperones UreE and UreG play important roles in the urease activation. In this study, we demonstrate that, in the presence of GTP and Mg(2+), UreG binds Ni(2+) with an affinity (Kd) of â ¼0.36 µm. The GTPase activity of Ni(2+)-UreG is stimulated by both K(+) (or NH4 (+)) and HCO3 (-) to a biologically relevant level, suggesting that K(+)/NH4 (+) and HCO3 (-) might serve as GTPase elements of UreG. We show that complexation of UreE and UreG results in two protein complexes, i.e. 2E-2G and 2E-G, with the former being formed only in the presence of both GTP and Mg(2+). Mutagenesis studies reveal that Arg-101 on UreE and Cys-66 on UreG are critical for stabilization of 2E-2G complex. Combined biophysical and bioassay studies show that the formation of 2E-2G complex not only facilitates nickel transfer from UreE to UreG, but also enhances the binding of GTP. This suggests that UreE might also serve as a structural scaffold for recruitment of GTP to UreG. Importantly, we demonstrate for the first time that UreE serves as a bridge to grasp Ni(2+) from HypA, subsequently donating it to UreG. The study expands our horizons on the molecular details of nickel translocation among metallochaperones UreE, UreG, and HypA, which further extends our knowledge on the urease maturation process. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 20 |
| Volume Number | 290 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2015-05-15 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacterial Proteins Chemistry Carrier Proteins GTP Phosphohydrolases Helicobacter Pylori Multiprotein Complexes Nickel Genetics Metabolism Biological Transport, Active Physiology Guanosine Triphosphate Mutagenesis Protein Binding Urease Research Support, Non-U.S. Gov't Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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