NDLI: Senescence-associated Long Non-coding RNA (SALNR) Delays Oncogene-induced Senescence through NF90 Regulation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wu, Cheng-lin Mao, Ze-bin Wang, Yu Jin, Bo Chen, Hao Xie, Bu-shan
Description	Author Affiliation: Wu CL ( From the Department of Biochemistry and Molecular Biology and Beijing Key Laboratory for 'Posttranslational Modification and Cellular Function,' Health Science Center, Peking University, 38 Xueyuan Road, Beijing 100191, China.); Wang Y ( the Departments of Urology and Microbiology, New York University Medical Center, New York, New York 10016, and.); Jin B ( From the Department of Biochemistry and Molecular Biology and Beijing Key Laboratory for 'Posttranslational Modification and Cellular Function,' Health Science Center, Peking University, 38 Xueyuan Road, Beijing 100191, China.); Chen H ( From the Department of Biochemistry and Molecular Biology and Beijing Key Laboratory for 'Posttranslational Modification and Cellular Function,' Health Science Center, Peking University, 38 Xueyuan Road, Beijing 100191, China.); Xie BS ( the Department of Gastroenterology, First Affiliated Hospital of Nanchang University, 17 Yongwai Zheng Street, Nanchang 330000, China.); Mao ZB ( From the Department of Biochemistry and Molecular Biology and Beijing Key Laboratory for 'Posttranslational Modification and Cellular Function,' Health Science Center, Peking University, 38 Xueyuan Road, Beijing 100191, China, zbmao@bjmu.edu.cn.)
Abstract	Long non-coding RNAs (lncRNAs) have recently emerged as key players in many physiologic and pathologic processes. Although many lncRNAs have been identified, few lncRNAs have been characterized functionally in aging. In this study, we used human fibroblast cells to investigate genome-wide lncRNA expression during cellular senescence. We identified 968 down-regulated lncRNAs and 899 up-regulated lncRNAs in senescent cells compared with young cells. Among these lncRNAs, we characterized a senescence-associated lncRNA (SALNR), whose expression was reduced during cellular senescence and in premalignant colon adenomas. Overexpression of SALNR delayed cellular senescence in fibroblast cells. Furthermore, we found that SALNR interacts with NF90 (nuclear factor of activated T-cells, 90 kDa), an RNA-binding protein suppressing miRNA biogenesis. We demonstrated that NF90 is a SALNR downstream target, whose inhibition led to premature senescence and enhanced expressions of senescence-associated miRNAs. Moreover, our data showed that Ras-induced stress promotes NF90 nucleolus translocation and suppresses its ability to suppress senescence-associated miRNA biogenesis, which could be rescued by SALNR overexpression. These data suggest that lncRNA SALNR modulates cellular senescence at least partly through changing NF90 activity.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	50
Volume Number	290
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2015-12-11
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Aging Genetics Nuclear Factor 90 Proteins Physiology Oncogenes RNA, Long Noncoding Cell Nucleolus Metabolism Cells, Cultured Genome-Wide Association Study Protein Transport Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in