NDLI: Tetranuclear ruthenium(II) complexes with oligo-oxyethylene linkers as one- and two-photon luminescent tracking non-viral gene vectors

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chao, Hui Huang, Huaiyi Qiu, Kangqiang Zhang, Pingyu Ji, Liangnian Yu, Bole
Description	Author Affiliation: Qiu K ( MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, 510275, P. R. China. ceschh@mail.sysu.edu.cn.)
Abstract	To prolong the observation time, increase the penetration depth and decrease self-absorption and phototoxicity, two-photon luminescent vectors have emerged as promising tools for tracking gene delivery in living cells. Herein, we report four new tetranuclear Ru(II) complexes based on oligo-oxyethylene and polybenzimidazole as one- and two- photon luminescent tracking non-viral gene vectors. In such a molecular design, the oligo-oxyethylene, polybenzimidazole and Ru(II) polypyridyl complexes were expected to render the vectors with increased cell permeability, biocompatibility, proton buffering capacity and one- and two-photon luminescence. Corresponding DNA interaction studies showed that the ability of the complexes to condense DNA decreased with increasing oligo-oxyethylene lengths. Additionally, all complexes protected DNA. The complexes were investigated as one- and two-photon tracking non-viral gene vectors in living cells and showed proper cellular uptake, good luciferase expression and low cytotoxicity.
ISSN	14779226
Issue Number	15
Volume Number	44
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2015-04-21
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Coordination Complexes Chemistry Genetic Vectors Ruthenium Benzimidazoles Cell Survival Drug Effects Pharmacology DNA Metabolism Ultrastructure Green Fluorescent Proteins Genetics HeLa Cells Humans Luminescence Microscopy, Confocal Microscopy, Electron, Transmission Photons Plasmids Polyethylene Glycols Polymers Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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