NDLI: Evidence of Native $Metal–S^{2−}–Metallothionein$ Complexes Confirmed by the Analysis of Cup1 Divalent-Metal-Ion Binding Properties

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Evidence of Native $Metal–S^{2−}–Metallothionein$ Complexes Confirmed by the Analysis of Cup1 Divalent-Metal-Ion Binding Properties

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Atrian, Sílvia Capdevila, Mercè Pagani, Ayelén Orihuela, Rubén Monteiro, Freddy
Description	Author Affiliation: Orihuela R ( Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.)
Abstract	It has previously been shown that recombinant synthesis, under metal-supplemented conditions, of diverse metallothioneins (MTs) results in the recovery of a subpopulation of $S^{2−}-containing$ complexes in addition to the $S^{2−}-devoid$ canonical metal–MT species. Further significance of this finding has remained veiled by the possibility of it being a mere consequence of synthesis in a heterologous bacterial system. Herein, we present definitive evidence that $S^{2−}$ ligands are also constituents of native metal–MT complexes. Because, although practically universal, the highest $S^{2−}$ content is incorporated by copper-thioneins when coordinating divalent metal ions, we adapted the Saccharomyces cerevisiae Cup1 protein, which is the most paradigmatic copper-thionein, as an experimental model. Most significantly, native Cd–Cup1 complexes were purified and fully spectroscopically and spectrometrically characterized from the 301N mutant yeast strain, which allows Cup1 synthesis even in the absence of copper. These results undoubtedly revealed the presence of a $Cd–S^{2−}–Cup1$ species in native preparations, which were only recovered when carefully avoiding the use of ion-exchange chromatography in the purification protocol. Furthermore, complete analysis of recombinant (Escherichia coli) Zn–Cup1, Cd–Cup1, and Cu–Cup1 and those complexes that result from Zn/Cd and Zn/Cu replacements in vitro and acidification/renaturalization processes yielded a comprehensive and comparative overview of the metal-binding abilities of Cup1. Overall, we consider the main conclusions of this study to go beyond the mere study of the particular Cup1 MT, so that they should be considered to delineate a new point of view on the interaction between copper-thioneins and divalent metal ions, still an unexplored aspect in MT research.
ISSN	09476539
e-ISSN	15213765
Journal	Chemistry - A European Journal
Issue Number	41
Volume Number	16
Language	English
Publisher	Wiley-VCH;ChemPubSoc Europe
Publisher Date	2010-11-02
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Copper Chemistry Metallothionein Sulfides Cadmium Circular Dichroism Escherichia Coli Metabolism Genetics Recombinant Proteins Saccharomyces Cerevisiae Zinc Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Catalysis

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