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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Torreggiani, Armida Atrian, Sílvia Capdevila, Mercè Domènech, Jordi Bofill, Roger Tinti, Anna |
| Description | Author Affiliation: Domènech J ( Departament de Genètica, Facultat de Biologia, Av. Diagonal 645, Universitat de Barcelona, E-08028, Barcelona, Spain.) |
| Abstract | Tetrahymena pyriformis MT1 (TpyMT1) is a model among ciliate metallothioneins (MTs). Here, we report on the analytic (ICP-AES, GC-FPD), spectroscopic (CD, UV–Vis, Raman) and spectrometric (ESI-MS) characterization of its recombinant Cd(II)-, Zn(II)- and Cu(I)-complexes, and of those formed during in vitro Zn/Cd and Zn/Cu replacement. In the presence of Cd(II), TpyMT1 renders a major Cd $_{11}$ –TpyMT1 species, which is also the final step reached in the in vitro Zn/Cd exchange process in Zn $_{11}$ –TpyMT1. Spectroscopic data supports a different folding of the isostoichiometric Cd $_{11}$ – and Zn $_{11}$ –TpyMT1 complexes. Unexpectedly, TpyMT1 biosynthesis in Zn(II)-rich cultures was sensitive to the aeration degree, so that high oxygenation rendered undermetalated, partially-oxidized, complexes (Zn $_{9}$ –TpyMT1). Biosynthesis in Cu(I)-rich media rendered extremely heterogeneous mixtures of Cu $_{x}$ Zn $_{y}$ -species ( x + y = 8–20), where the higher the aeration, the higher the Zn(II) content. The complexity of these samples was reproduced during the Zn/Cu replacement, as the number of generated species increased gradually with the addition of copper to Zn $_{11}$ –TpyMT1. According to our results, a clear preference of TpyMT1 for Cd(II) binding, rather than for Zn(II), and especially Cu(I) can be postulated. This character is totally consistent with the induction pattern of the TpyMT1 gene and the postulated role of TpyMT1 in Cd-detoxification. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 4 |
| Volume Number | 1784 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-04-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Metallothionein Chemistry Metals, Heavy Metabolism Protozoan Proteins Tetrahymena Pyriformis Animals Cadmium Circular Dichroism Copper Protein Binding Protein Structure, Tertiary Spectrum Analysis, Raman Zinc Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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