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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chen, Peilin Charan, Shobhit Singh, Narendra Kuo, Chiung-wen Chien, Fan-ching |
| Description | Author Affiliation: Charan S ( Research Center For Applied Sciences, Academia Sinica, Taipei-115, Taiwan.) |
| Abstract | A simple, sensitive, and highly specific lipid targeting Raman probe (Nile red coated silver nanoparticles) has been developed to image living nematode Caenorhabditis elegans (C. elegans). Our idea of imaging lipids in C. elegans is to combine the specificity of the fluorescent dye, Nile red, and the highly enhanced Raman scattering on the silver nanoparticles. Our strategy involves the fabrication of a lipid targeting probe, which is incorporated into the intracellular intestinal granules of C. elegans by incubating these worms in the solution containing Raman probes, resulting in an uptake and subsequent incorporation of these Raman probes into the intestinal granule, thus allowing fast visualization of lipid droplets through a conventional confocal imaging technique. |
| ISSN | 09476539 |
| e-ISSN | 15213765 |
| Journal | Chemistry - A European Journal |
| Issue Number | 18 |
| Volume Number | 17 |
| Language | English |
| Publisher | Wiley-VCH;ChemPubSoc Europe |
| Publisher Date | 2011-04-26 |
| Publisher Place | Germany |
| Access Restriction | Open |
| Subject Keyword | Caenorhabditis Elegans Metabolism Fluorescent Dyes Chemical Synthesis Lipids Animals Coloring Agents Escherichia Coli Chemistry Microscopy, Confocal Nanoparticles Ultrastructure Oxazines Spectrophotometry, Ultraviolet Spectrum Analysis, Raman Research Support, Non-U.S. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Catalysis |
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