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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Takamura, Naohide Kashiwada, Ayumi Brandenburg, Enrico Matsuda, Kiyomi Koksch, Beate Tsuboi, Mana |
| Description | Author Affiliation: Kashiwada A ( Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University, Narashino, Chiba 275-8575, Japan. kashiwada.ayumi@nihon-u.ac.jp) |
| Abstract | A weakly acidic pH-responsive polypeptide is believed to have the potential for an endosome escape function in a polypeptide-triggered delivery system. For constructing a membrane fusion device with pH-responsiveness, we have designed novel polypeptides that are capable of forming an α2 coiled coil structure. Circular dichroism spectroscopy reveals that a polypeptide, AP-LZ(EH5), with a Glu and His salt-bridge pair at a staggered position in the hydrophobic core forms a stable coiled coil structure only at endosomal pH values (pH 5.0 to 5.5). On the basis of their endosomal-pH responsiveness, a boronic acid/polypeptide conjugate (BA-H5-St) was also designed as a pilot molecule to construct a pH-responsive, one-way membrane fusion system with a sugarlike compound (phosphatidylinositol: PI)-containing liposome as a target. Membrane fusion behavior was characterized by lipid-mixing, inner-leaflet lipid-mixing, and contents-mixing assays. These studies reveal that membrane fusion is clearly observed when the pH of the experimental system is changed from 7.4 (physiological condition) to 5.0 (endosomal condition). |
| ISSN | 09476539 |
| e-ISSN | 15213765 |
| Journal | Chemistry - A European Journal |
| Issue Number | 22 |
| Volume Number | 17 |
| Language | English |
| Publisher | Wiley-VCH;ChemPubSoc Europe |
| Publisher Date | 2011-05-23 |
| Publisher Place | Germany |
| Access Restriction | Open |
| Subject Keyword | Boronic Acids Chemistry Endosomes Glutamic Acid Liposomes Peptides Circular Dichroism Hydrogen-Ion Concentration Membrane Fusion Membranes, Artificial Molecular Sequence Data Molecular Structure Thermodynamics Research Support, Non-U.S. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Catalysis |
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