NDLI: Detection of Single Nucleotide Polymorphisms by a Gold Nanowire-on-Film SERS Sensor Coupled with S1 Nuclease $Treatment^{†}$

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Bongsoo Lee, Sang Yup Kang, Taejoon Yoo, Seung Min
Description	Author Affiliation: Yoo SM ( Department of Chemical and Biomolecular, Engineering (BK21 Program), KAIST, Daejeon 305-701, Korea.)
Abstract	Single nucleotide polymorphisms (SNPs) can serve as important biomarkers for genetic diseases, for which accurate detection of SNPs is essential for early diagnosis. We have developed a novel SNP sensor by combining a Au nanowire-on-film surface-enhanced Raman scattering (SERS) platform with S1 nuclease reaction. The combined sensor system provides reproducible SERS signals only in the presence of perfectly matched target DNAs, to probe DNAs as a result of single-stranded DNA-specific degradation by S1 nuclease. Furthermore, point mutations in DNA causing Wilson disease and Avellino corneal dystrophy were successfully identified by this sensor, thereby indicating its practical ability to diagnose genetic diseases.
ISSN	09476539
e-ISSN	15213765
Journal	Chemistry - A European Journal
Issue Number	31
Volume Number	17
Language	English
Publisher	Wiley-VCH;ChemPubSoc Europe
Publisher Date	2011-07-25
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	DNA Chemistry Endonucleases Metabolism Polymorphism, Single Nucleotide Genetics Metal Nanoparticles Spectrum Analysis, Raman Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Catalysis

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Detection of Single Nucleotide Polymorphisms by a Gold Nanowire-on-Film SERS Sensor Coupled with S1 Nuclease $Treatment^{†}$