NDLI: Phospholipid–Detergent Conjugates as Novel Tools for siRNA Delivery

Please wait, while we are loading the content...

Year: 2017 Volume: 23

Year: 2016 Volume: 22

Year: 2015 Volume: 21

Year: 2014 Volume: 20

Year: 2013 Volume: 19

Year: 2013 Volume: 19 Issue: 52

Year: 2013 Volume: 19 Issue: 51

Year: 2013 Volume: 19 Issue: 50

Year: 2013 Volume: 19 Issue: 49

Year: 2013 Volume: 19 Issue: 48

Year: 2013 Volume: 19 Issue: 47

Year: 2013 Volume: 19 Issue: 46

Year: 2013 Volume: 19 Issue: 45

Year: 2013 Volume: 19 Issue: 44

Year: 2013 Volume: 19 Issue: 43

Year: 2013 Volume: 19 Issue: 42

Year: 2013 Volume: 19 Issue: 41

Year: 2013 Volume: 19 Issue: 40

Year: 2013 Volume: 19 Issue: 39

Year: 2013 Volume: 19 Issue: 38

Year: 2013 Volume: 19 Issue: 37

Year: 2013 Volume: 19 Issue: 36

Year: 2013 Volume: 19 Issue: 35

Year: 2013 Volume: 19 Issue: 34

Year: 2013 Volume: 19 Issue: 33

Year: 2013 Volume: 19 Issue: 32

Year: 2013 Volume: 19 Issue: 31

Year: 2013 Volume: 19 Issue: 30

Year: 2013 Volume: 19 Issue: 29

Year: 2013 Volume: 19 Issue: 28

Year: 2013 Volume: 19 Issue: 27

Year: 2013 Volume: 19 Issue: 26

Year: 2013 Volume: 19 Issue: 25

Year: 2013 Volume: 19 Issue: 24

Year: 2013 Volume: 19 Issue: 23

Year: 2013 Volume: 19 Issue: 22

Year: 2013 Volume: 19 Issue: 21

Year: 2013 Volume: 19 Issue: 20

Year: 2013 Volume: 19 Issue: 19

Year: 2013 Volume: 19 Issue: 18

Year: 2013 Volume: 19 Issue: 17

Year: 2013 Volume: 19 Issue: 16

Year: 2013 Volume: 19 Issue: 15

Year: 2013 Volume: 19 Issue: 14

Year: 2013 Volume: 19 Issue: 13

Year: 2013 Volume: 19 Issue: 12

Year: 2013 Volume: 19 Issue: 11

Year: 2013 Volume: 19 Issue: 10

Year: 2013 Volume: 19 Issue: 9

Year: 2013 Volume: 19 Issue: 8

Year: 2013 Volume: 19 Issue: 7

Alkaline Earth Metal Ion Induced Coil–Helix–Coil Transition of Lysine–Coumarin–Azacrown Hybrid Foldamers with OFF–OFF–ON Fluorescence Switching

Synthesis of 2′-Deoxy-1′-homo-N-nucleosides with Anti-Influenza Activity by Catalytic Methyltrioxorhenium $(MTO)/H_{2}O_{2}$ Oxyfunctionalization

Highly Sensitive and Selective Spectroscopic Detection of Mercury(II) in Water by Using Pyridylporphyrin–DNA Conjugates

Fine-Tuning the Reactivity and Stability by Systematic Ligand Variations in $CpCo^{I}$ Complexes as Catalysts for [2+2+2] Cycloaddition Reactions

$Ag/AgBr/Co–Ni–NO_{3}$ Layered Double Hydroxide Nanocomposites with Highly Adsorptive and Photocatalytic Properties

Asymmetric Hydrogenation Using Rhodium Complexes Generated from Mixtures of Monodentate Neutral and Anionic Phosphorus Ligands

Hierarchical Self-Assembly of a Biomimetic Light-Harvesting Antenna Based on DNA G-Quadruplexes

Rhodium-Catalyzed [2+2+2] Cycloaddition of Oximes and Diynes To Give Pyridines

Synthesis, Structure, and Single-Molecule Magnetic Properties of Rare-Earth Sandwich Complexes with Mixed Phthalocyanine and Schiff Base Ligands

An Unsymmetrically π-Extended Porphyrin-Based Single-Crystal Field-Effect Transistor and Its Anisotropic Carrier-Transport Behavior

A Modular Synthesis of Teraryl-Based α-Helix Mimetics, Part 2: Synthesis of 5-Pyridine Boronic Acid Pinacol Ester Building Blocks with Amino Acid Side Chains in 3-Position

Microenvironment Effects in Electrocatalysis: Ionic-Liquid-Like Coating on Carbon Nanotubes Enhances the Pd-Electrocatalytic Alcohol Oxidation

TetrathiafulvaleneBenzothiadiazoles as Redox-Tunable Donor–Acceptor Systems: Synthesis and Photophysical Study

A Modular Synthesis of Teraryl-Based α-Helix Mimetics, Part 1: Synthesis of Core Fragments with Two Electronically Differentiated Leaving Groups

Palladium-Catalyzed Intermolecular Desulfinylative Cross-Coupling of Heteroaromatic Sulfinates

Anodic-Stripping Voltammetric Immunoassay for Ultrasensitive Detection of Low-Abundance Proteins Using Quantum Dot Aggregated Hollow Microspheres

Design and Synthesis of a New Family of Fluorinated Liquid Crystals

Isomerization Energy Decomposition Analysis for Highly Ionic Systems: Case Study of Starlike $E_{5}Li_{7}^{+}$ Clusters

Collective Total Synthesis of Englerin A and B, Orientalol E and F, and Oxyphyllol: Application of the Organocatalytic [4+3] Cycloaddition Reaction

Inner Workings of a Cinchona Alkaloid Catalyzed Oxa-Michael Cyclization: Evidence for a Concerted Hydrogen-Bond-Network $Mechanism^{†}$

Syntheses and Characterization of New Vinyl-Borylene Complexes by the Hydroboration of Alkynes with $[(μ_{3}-BH)(Cp*RuCO)_{2}(μ-CO)Fe(CO)_{3}]^{†}$

Facile Electrochemical Oxidation of Polyaromatic Hydrocarbons to Surface-Confined Redox-Active Quinone Species on a Multiwalled Carbon Nanotube Surface

Generation of Molecular Complexity from Cyclooctatetraene: Preparation of Optically Active Protected Aminocycloheptitols and Bicyclo[4.4.1]undecatriene

An Efficient Method for Sterically Demanding Suzuki–Miyaura Coupling Reactions

Phospholipid–Detergent Conjugates as Novel Tools for siRNA Delivery

$[(η^{6}-Praziquantel)Cr(CO)_{3}]$ Derivatives with Remarkable In Vitro Anti-schistosomal Activity

Diastereoselective Access to Polyoxygenated Polycyclic Spirolactones through a Rhodium-Catalyzed [3+2] Cycloaddition Reaction: Experimental and Theoretical Studies

$C_{5}Li_{7}^{+}$ and $O_{2}Li_{5}^{+}$ as Noble-Gas-Trapping Agents

The Hydride-Ion Affinity of Borenium Cations and Their Propensity to Activate $H_{2}$ in Frustrated Lewis Pairs

A New Modular Phosphite-Pyridine Ligand Library for Asymmetric Pd-Catalyzed Allylic Substitution Reactions: A Study of the Key Pd-π-Allyl Intermediates

S-Alkylation of Soft Scorpionates

Gold(I) and Silver(I) Complexes of Diphosphacyclobutadiene Cobaltate Sandwich Anions

Cross-Linked Helically Chiral Poly-(γ-benzyl-L-glutamate) as Enantiodiscriminating Alignment Medium

Synthesis and Thermoreversible Gelation Properties of Main-Chain Poly(pyridine-2,6-dicarboxamide-triazole)s

Ionic-Liquid-Assisted Synthesis of Uniform Fluorinated B/C-Codoped $TiO_{2}$ Nanocrystals and Their Enhanced Visible-Light Photocatalytic Activity

Electrochemical Deposition of Mesoporous Pt–Au Alloy Films in Aqueous Surfactant Solutions: Towards a Highly Sensitive Amperometric Glucose Sensor

Cascade Syntheses Routes to the Centrocountins

Nitrogen-Doped Carbon Dots: A Facile and General Preparation Method, Photoluminescence Investigation, and Imaging Applications

Synthesis and Morphogenesis of Organic and Inorganic Polymers by Means of Biominerals and Biomimetic Materials

Stereoselective Multiple Bond-Forming Transformations (MBFTs): The Power of 1,2- and 1,3-Dicarbonyl Compounds

Year: 2013 Volume: 19 Issue: 6

Year: 2013 Volume: 19 Issue: 5

Year: 2013 Volume: 19 Issue: 4

Year: 2013 Volume: 19 Issue: 3

Year: 2013 Volume: 19 Issue: 2

Year: 2013 Volume: 19 Issue: 1

Year: 2012 Volume: 18

Year: 2011 Volume: 17

Year: 2010 Volume: 16

Year: 2009 Volume: 15

Year: 2008 Volume: 14

Year: 2007 Volume: 13

Year: 2006 Volume: 12

Year: 2005 Volume: 11

Year: 1997 Volume: 3

Phospholipid–Detergent Conjugates as Novel Tools for siRNA Delivery

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Strub, Jean-marc Pierrat, Philippe Lebeau, Luc Creusat, Gaëlle Zuber, Guy Laverny, Gilles Wehrung, Patrick Pons, Françoise Vandorsselaer, Alain
Description	Author Affiliation: Pierrat P ( Laboratoire de Conception et Application de Molécules Bioactives, CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch, France.)
Abstract	One of the potential benefits of drug delivery systems in medicine is the creation of nanoparticle-based vectors that deliver a therapeutic cargo in sufficient quantity to a target site to enable a selective effect, width of the therapeutic window depending on the toxicity of the vector and the cargo. In this work, we intended to improve the siRNA delivery efficiency of a new kind of nucleic acid carrier, which is the result of the conjugation of the membrane phospholipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) to the membrane-active species Triton X-100 (TX100). We hypothesized that by improving the biodegradability the cytotoxicity of the conjugate might by reduced, whereas its original transfection potential would be tentatively preserved. DOPC was conjugated to Triton X-100 through spacers displaying various resistance to chemical hydrolysis and enzyme degradation. The results obtained through in vitro siRNA delivery experiments showed that the initial phosphoester bond can be replaced with a phospho(alkyl)enecarbonate group with no loss in the transfection activity, whereas the associated cytotoxicity was significantly decreased, as assessed by metabolic activity and membrane integrity measurements. The toxicity of the conjugates incorporating a phospho(alkyl)enesuccinnate moiety proved even lower but was clearly balanced with a reduction of the siRNA delivery efficiency. Hydrolytic stability and intracellular degradation of the conjugates were investigated by NMR spectroscopy and mass spectrometry. A general trend was that the more readily degraded conjugates were those with the lower toxicity. Otherwise, the phospho(alkyl)enecarbonate conjugates revealed some hemolytic activity, whereas the parent phosphoester did not. The reason why these conjugates behave differently with respect to hemolysis might be a consequence of unusual fusogenic properties and probably reflects the difference in the stability of the conjugates in the intracellular environment.
ISSN	09476539
e-ISSN	15213765
Journal	Chemistry - A European Journal
Issue Number	7
Volume Number	19
Language	English
Publisher	Wiley-VCH;ChemPubSoc Europe
Publisher Date	2013-02-11
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Detergents Chemistry Pharmacology Glycerylphosphorylcholine Analogs & Derivatives Nanoparticles Nucleic Acids Phospholipids Phosphorylcholine RNA, Small Interfering Cell Line, Tumor Drug Delivery Systems Magnetic Resonance Spectroscopy Mass Spectrometry Metabolism Transfection Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Catalysis

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in