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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, Wenying Jiao, Huping Chen, Yang Chen, Jian Yu, Cong Wang, Yan Zhang, Qingfeng |
| Description | Author Affiliation: Wang Y ( State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 (P.R. China)) |
| Abstract | A new label-free fluorescence turn-on strategy for highly sensitive biosensing has been developed. A negatively charged perylene probe was synthesized. Polycations could induce aggregation of the perylene probe through noncovalent interactions and the fluorescence of the probe’s monomer was efficiently quenched. Upon addition of a single-stranded nucleic acid, competitive binding of the negatively charged nucleic acid (a polyanion) to the cationic polymer resulted in the release of a monomer and thus a turn-on fluorescence signal was detected. Without the use of any amplification techniques, a detection limit of 2 pM DNA was obtained. Based on these results, an assay strategy for the highly sensitive detection of alkaline phosphatase (ALP) activity has been demonstrated. λ Exonuclease (λ exo) could degrade 5′-phosphorylated single-stranded DNA. However, when the DNA sample was treated with ALP, the phosphate functional group was removed by ALP and it could no longer be degraded by λ exo. Binding of the DNA to the perylene probe–polycation complex resulted in a turn-on fluorescence signal, which could be used for sensing of ALP. The method is highly sensitive, a limit of detection as low as $0.02 mU mL^{−1}$ ALP was obtained. Our method is simple, convenient, highly sensitive, and inexpensive. |
| ISSN | 09476539 |
| e-ISSN | 15213765 |
| Journal | Chemistry - A European Journal |
| Issue Number | 38 |
| Volume Number | 19 |
| Language | English |
| Publisher | Wiley-VCH;ChemPubSoc Europe |
| Publisher Date | 2013-09-16 |
| Publisher Place | Germany |
| Access Restriction | Open |
| Subject Keyword | Biosensing Techniques DNA, Single-Stranded Chemistry Fluorescent Dyes Perylene Polymers Alkaline Phosphatase Hydrogen Bonding Hydrogen-Ion Concentration Research Support, Non-U.S. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Catalysis |
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