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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Demartini, Diogo Ribeiro Fragoso, Rodrigo R. Stanisçuaski, Fernanda Verli, Hugo Schroeder, Evelyn Koeche Mulinari, Fernanda Ligabue-braun, Rodrigo Becker-ritt, Arlete Beatriz Grossi-de-sá, Maria Fátima Carlini, Célia Regina |
| Description | Author Affiliation: Mulinari F ( Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. fermulinari@pq.cnpq.br) |
| Abstract | Ureases, nickel-dependent enzymes that catalyze the hydrolysis of urea into ammonia and bicarbonate, are widespread in plants, bacteria, and fungi. Previously, we cloned a cDNA encoding a Canavalia ensiformis urease isoform named JBURE-II, corresponding to a putative smaller urease protein (78 kDa) when compared to other plant ureases. Aiming to produce the recombinant protein, we obtained jbure - IIb , with different 3′ and 5′ ends, encoding a 90 kDa urease. Three peptides unique to the JBURE-II/-IIb protein were detected by mass spectrometry in seed extracts, indicating that jbure - II /- IIb is a functional gene. Comparative modeling indicates that JBURE-IIb urease has an overall shape almost identical to C . ensiformis major urease JBURE-I with all residues critical for urease activity. The cDNA was cloned into the pET101 vector and the recombinant protein was produced in Escherichia coli . The JBURE-IIb protein, although enzymatically inactive presumably due to the absence of Ni atoms in its active site, impaired the growth of a phytopathogenic fungus and showed entomotoxic properties, inhibiting diuresis of Rhodnius prolixus isolated Malpighian tubules, in concentrations similar to those reported for JBURE-I and canatoxin. The antifungal and entomotoxic properties of the recombinant JBURE-IIb apourease are consistent with a protective role of ureases in plants. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 12 |
| Volume Number | 1814 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-12-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Canavalia Enzymology Genetics Urease Amino Acid Sequence Antifungal Agents Chemistry Isolation & Purification Metabolism Pharmacology Cloning, Molecular Isoenzymes Microbial Sensitivity Tests Molecular Sequence Data Mutagenesis, Site-Directed Phylogeny Plant Proteins Recombinant Proteins Sequence Homology, Nucleic Acid Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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