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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Croce, Roberta Wientjes, Emilie Roest, Gemma |
| Description | Author Affiliation: Wientjes E ( Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.) |
| Abstract | The first event of photosynthesis is the harvesting of solar energy by a large array of pigments. These pigments are coordinated to proteins that organize them to assure efficient excitation energy transfer. The protein plays an essential role in tuning the spectroscopic properties of the pigments, by determining their site energy and/or by favoring pigment–pigments interactions. Here we investigate how the protein modulates the pigment properties by using a single-point-mutation approach. We monitor changes in the low-energy absorption/emission band of Lhca4, which is well separated from the bulk absorption and thus represents an attractive model system. Moreover, it was recently shown that Lhca4 exists in at least two conformations, a dominating one emitting at 720 nm and a second one emitting at 685 nm (Kruger et al. PNAS 2011). Here we show that a single amino-acid substitution (from Asn to Gln, which are both chlorophyll-binding residues and only differ for one C―C bond), moves the equilibrium almost completely towards the 685-nm conformation. This indicates that small changes in the protein can have a large effect on the properties of the pigments. We show that His99, which was suggested to coordinate a red-absorbing chlorophyll (Melkozernov and Blankenship, JBC 2003), is not a chlorophyll ligand. We also show that single amino-acid substitutions nearby the chlorophylls allow to tune the emission spectrum of the pigments over a wide range of wavelengths and to modulate the excited-state lifetimes of the complex. These findings are discussed in the light of previously proposed non-photochemical quenching models. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 5 |
| Volume Number | 1817 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-05-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Arabidopsis Proteins Metabolism Arabidopsis Radiation Effects Chlorophyll Binding Proteins Photosystem I Protein Complex Pigments, Biological Absorption Chemistry Genetics Circular Dichroism Kinetics Models, Molecular Mutagenesis Mutation Quantum Theory Spectrometry, Fluorescence Time Factors Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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