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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sherman, Louis A. Whitmarsh, John Wang, Qing Jun Nedbal, Ladislav Li, Hong Singh, Abhay |
| Description | Author Affiliation: Wang QJ ( Center for Biophysics & Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. qingjun.wang@uky.edu) |
| Abstract | Oxygenic photosynthesis in cyanobacteria, algae, and plants requires photosystem II (PSII) to extract electrons from H $_{2}$ O and depends on photosystem I (PSI) to reduce NADP $^{+}$ . Here we demonstrate that mixotrophically-grown mutants of the cyanobacterium Synechocystis sp. PCC 6803 that lack PSI (ΔPSI) are capable of net light-induced O $_{2}$ evolution in vivo . The net light-induced O $_{2}$ evolution requires glucose and can be sustained for more than 30 min. Utilizing electron transport inhibitors and chlorophyll a fluorescence measurements, we show that in these mutants PSII is the source of the light-induced O $_{2}$ evolution, and that the plastoquinone pool is reduced by PSII and subsequently oxidized by an unidentified electron acceptor that does not involve the plastoquinol oxidase site of the cytochrome b $_{6}$ f complex. Moreover, both O $_{2}$ evolution and chlorophyll a fluorescence kinetics of the ΔPSI mutants are highly sensitive to KCN, indicating the involvement of a KCN-sensitive enzyme(s). Experiments using $^{14}$ C-labeled bicarbonate show that the ΔPSI mutants assimilate more CO $_{2}$ in the light compared to the dark. However, the rate of the light-minus-dark CO $_{2}$ assimilation accounts for just over half of the net light-induced O $_{2}$ evolution rate, indicating the involvement of unidentified terminal electron acceptors. Based on these results we suggest that O $_{2}$ evolution in ΔPSI cells can be sustained by an alternative electron transport pathway that results in CO $_{2}$ assimilation and that includes PSII, the platoquinone pool, and a KCN-sensitive enzyme. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 5 |
| Volume Number | 1817 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-05-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Gene Deletion Oxygen Metabolism Photosystem I Protein Complex Genetics Synechocystis Carbon Dioxide Cell Respiration Drug Effects Radiation Effects Chlorophyll Cytochrome B6f Complex Dibromothymoquinone Pharmacology Diuron Fluorescence Glucose Kinetics Oxidation-Reduction Photosystem II Protein Complex Plastoquinone Potassium Cyanide Sodium Bicarbonate Spectrum Analysis Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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