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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lin, Xin Parthasarathy, Krupakar Zhang, Tong Mu, Yuguang Surya, Wahyu Torres, Jaume |
| Description | Author Affiliation: Lin X ( School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, 637551, Singapore.); Parthasarathy K ( School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, 637551, Singapore.); Surya W ( School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, 637551, Singapore.); Zhang T ( School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, 637551, Singapore.); Mu Y ( School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, 637551, Singapore.); Torres J ( School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, 637551, Singapore. Electronic address: jtorres@ntu.edu.sg.) |
| Abstract | Crystal (Cry) toxins are widely used for insect control, but their mechanism of toxicity is still uncertain. These toxins can form lytic pores in vitro, and water soluble tetrameric pre-pore intermediates have been reported. Even the precise oligomeric state of the toxin in membranes, trimeric or tetrameric, is still a debated issue. Based on previous reports, we have assumed that interactions between toxin monomers in solution are at least partly mediated by domain I, and we have analyzed in silico the homo-oligomerization tendencies of the domain I α-helices individually. Using many homologous sequences for each α-helix, our strategy allows selection of evolutionarily conserved interactions. These interactions appeared only in helices α3 and α5, but only α3 produced a suitably oriented or α-helical sample in lipid bilayers, forming homotetramers in C14-betaine, and allowing determination of its rotational orientation in lipid bilayers using site-specific infrared dichroism (SSID). The determined orientation in the tetrameric model is in agreement with only one of the evolutionarily conserved models. In addition mutation R99E, which was found to inhibit oligomerization experimentally, greatly destabilized the tetramer in molecular dynamic simulations. In this model, helix 3 is able to form inter-monomer interactions without significant rearrangements of domain I, which is compatible with the available crystal structure of Cry toxins in solution. The model presented here at least partially explains the reported tetrameric oligomerization of Cry toxins in solution and the inhibition of this oligomerization by a synthetic α3 peptide. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 7 |
| Volume Number | 1838 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-07-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Bacterial Proteins Chemistry Endotoxins Hemolysin Proteins Insecticides Lipid Bilayers Amino Acid Sequence Cell Membrane Models, Molecular Molecular Sequence Data Protein Binding Protein Structure, Secondary Protein Structure, Tertiary Sequence Alignment Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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