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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bellard, Elisabeth Golzio, Muriel Sébaï, Sarra C. Rols, Marie-pierre Teissié, Justin Escoffre, Jean-michel Faurie, Cécile |
| Description | Author Affiliation: Escoffre JM ( CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077, Toulouse, France); Bellard E ( CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077, Toulouse, France); Faurie C ( Matwin-Institut Bergonié, 229 cours de l'Argonne, 33076 Bordeaux cedex, France.); Sébaï SC ( Eviagenics, Immeuble Villejuif Biopark, 1 Mail du Professeur Georges Mathé, 94800 Villejuif, France.); Golzio M ( CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077, Toulouse, France); Teissié J ( CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077, Toulouse, France); Rols MP ( CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, F-31077, Toulouse, France) |
| Abstract | Membrane electropermeabilization relies on the transient permeabilization of the plasma membrane of cells submitted to electric pulses. This method is widely used in cell biology and medicine due to its efficiency to transfer molecules while limiting loss of cell viability. However, very little is known about the consequences of membrane electropermeabilization at the molecular and cellular levels. Progress in the knowledge of the involved mechanisms is a biophysical challenge. As a transient loss of membrane cohesion is associated with membrane permeabilization, our main objective was to detect and visualize at the single-cell level the incidence of phospholipid scrambling and changes in membrane order. We performed studies using fluorescence microscopy with C6-NBD-PC and FM1-43 to monitor phospholipid scrambling and membrane order of mammalian cells. Millisecond permeabilizing pulses induced membrane disorganization by increasing the translocation of phosphatidylcholines according to an ATP-independent process. The pulses induced the formation of long-lived permeant structures that were present during membrane resealing, but were not associated with phosphatidylcholine internalization. These pulses resulted in a rapid phospholipid flip/flop within less than 1 s and were exclusively restricted to the regions of the permeabilized membrane. Under such electrical conditions, phosphatidylserine externalization was not detected. Moreover, this electrically-mediated membrane disorganization was not correlated with loss of cell viability. Our results could support the existence of direct interactions between the movement of membrane zwitterionic phospholipids and the electric field. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 7 |
| Volume Number | 1838 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-07-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Cell Membrane Metabolism Phospholipids Adenosine Triphosphate Animals CHO Cells Cell Line Cell Membrane Permeability Cell Survival Physiology Cricetulus Electroporation Phosphatidylcholines Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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