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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Losick, R. Tjian, R. |
| Abstract | The activity of the sigma subunit of Bacillus subtilis RNA polymerase decreases markedly during the first hours of sporulation [T.G. Linn et al. (1973) Proc. Nat. Acad. Sci. USA 70, 1865-1869]. We have prepared antibody against RNA polymerase holoenzyme to determine the fate of sigma polypeptide during spore formation. This antiserum specifically and independently precipitates sigma and core polymerase from crude extracts of B. subtilis as judged by both sodium dodecyl sulfate and urea gel electrophoresis of the precipitates. We report that crude extracts of sporulating cells lacking sigma activity contain as much sigma polypeptide as extracts of vegetative cells. However, sigma polypeptide in extracts from sporulating cells is apparently only weakly associated with RNA polymerase, as indicated by the failure of sigma to co-purify efficiently with core enzyme during phase partitioning. The loss of sigma activity and the weak binding of sigma to core enzyme occurs normally in a mutant blocked at an intermediate stage of sporulation (SpoII-4Z) and in wild-type bacteria sporulating in 121B medium, Difco sporulation medium, or Sterlini-Mandelstam resuspension medium. In contrast, sigma in two mutants (SpoOa-5NA and SpoOb-6Z) blocked at an early stage of spore formation remains active and tightly associated with RNA polymerase during stationary phase. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 7 |
| Volume Number | 71 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1974-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacillus Subtilis Enzymology DNA-Directed RNA Polymerases Spores, Bacterial Animals Antibodies Electrophoresis, Polyacrylamide Gel Mutation Precipitin Tests Rabbits Immunology Sodium Dodecyl Sulfate Transcription, Genetic Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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