NDLI: Novel RNA polymerase sigma factor from Bacillus subtilis.

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Regulation of expression from the glnA promoter of Escherichia coli in the absence of glutamine synthetase.

Intracellular injection of t he catalytic subunit of cyclic AMP-dependent protein kinase simulates facilitation of transmitter release underlying behavioral sensitization in Aplysia.

Construction and identification of bacterial plasmids containing nucleotide sequence for human leukocyte interferon.

Unusual sequence homology at the 5-ends of the developmentally regulated beta A-, beta C-, and gamma-globin genes of the goat.

Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.

Allelic forms of beta 2-microglobulin in the mouse.

Anti-AMP antibody precipitation of multiply adenylylated forms of glutamine synthetase from Escherichia coli: a model relating both concentration and density of antigenic sites with the antibody-antigen interaction.

Analysis of the mechanism of fast axonal transport by intracellular injection of potentially inhibitory macromolecules: evidence for a possible role of actin filaments.

Dopamine and depolarizing agents regulate the state of phosphorylation of protein I in the mammalian superior cervical sympathetic ganglion.

Regulation by guanosine 3':5'-cyclic monophosphate of phospholipid methylation during chemotaxis in Dictyostelium discoideum.

Organization of unc gene cluster of Escherichia coli coding for proton-translocating ATPase of oxidative phosphorylation.

Cell-free coupled transcription-translation system for investigation of linear DNA segments.

Monoclonal antibodies to beta-adrenergic receptors: use in purification and molecular characterization of beta receptors.

Affinity labeling of rat liver thyroid hormone nuclear receptor.

Presence of a flavin semiquinone in methanol oxidase.

Translation and developmental regulation of RNA encoded by the eukaryotic transposable element copia.

Regulation of herpesvirus macromolecular synthesis: transcription-initiation sites and domains of alpha genes.

OK10, an avian acute leukemia virus of the MC29 subgroup with a unique genetic structure

A cyclic enkephalin analog with high in vitro opiate activity.

Macromolecular photoaffinity labeling of the lutropin receptor on granulosa cells.

A change in the rate of transcription of a eukaryotic gene in response to cyclic AMP.

Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903.

Kinetic aspects of cycloheximide-induced reversal of adrenocorticotropin effects on steroidogenesis and adrenal phospholipids in vivo.

Isolation and characterization of a Pseudomonas aeruginosa mutant producing a nontoxic, immunologically crossreactive toxin A protein.

Alteration of lymphocyte surface properties by insertion of foreign functional components of plasma membrane.

Distribution of virus structural proteins and protein-protein interactions in plasma membrane of baby hamster kidney cells infected with Sindbis or vesicular stomatitis virus.

Reversible growth arrest in simian virus 40-transformed human fibroblasts.

Molecular evidence for genetic exchanges among ribosomal genes on nonhomologous chromosomes in man and apes.

No apparent nucleotide sequence specificity in cellular DNA juxtaposed to retrovirus proviruses.

Genetic transformation of mouse embryos by microinjection of purified DNA.

Anti-alprenolol anti-idiotypic antibodies bind to beta-adrenergic receptors and modulate catecholamine-sensitive adenylate cyclase.

H-2 restriction as a consequence of intentional priming: T cells of fully allogeneic chimeric mice as well as of normal mice respond to foreign antigens in the context of H-2 determinants not encountered on thymic epithelial cells.

Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma

Adrenergic regulation of intraocular pressure: identification of beta 2-adrenergic-stimulated adenylate cyclase in ciliary process epithelium.

Identification of human eosinophil lysophospholipase as the constituent of Charcot-Leyden crystals.

The structural polypeptides of aphthovirus are phosphoproteins.

[3H]Phencyclidine: a probe for the ionic channel of the nicotinic receptor.

Barbiturate receptor sites are coupled to benzodiazepine receptors.

Microinjection of catalytic subunit of cyclic AMP-dependent protein kinase enhances calcium action potentials of bag cell neurons in cell culture.

Cloning and complete nucleotide sequence of human 5'-alpha-globin gene.

Isolation and characterization of overlapping genomic clones covering the chicken alpha 2 (type I) collagen gene.

Regulation of myosin self-assembly: phosphorylation of Dictyostelium heavy chain inhibits formation of thick filaments.

Novel RNA polymerase sigma factor from Bacillus subtilis.

DNA polymerase alpha mutants from a Drosophila melanogaster cell line.

Receptor structure in the bacterial sensing system.

Specific disruption of vimentin filament organization in monkey kidney CV-1 cells by diphtheria toxin, exotoxin A, and cycloheximide.

Molecular analysis of a gene in a developmentally regulated puff of Drosophila melanogaster.

Molecular limits on the size of a genetic locus in Drosophila melanogaster.

Transcripts of the immunoglobulin C mu gene vary in structure and splicing during lymphoid development.

Mouse immunoglobulin D: construction and characterization of a cloned delta chain cDNA.

Assembly of subcomponents C1r and C1s of first component of complement: electron microscopic and ultracentrifugal studies.

Release of 1-O-alkylglyceryl 3-phosphorylcholine, O-deacetyl platelet-activating factor, from leukocytes: chemical ionization mass spectrometry of phospholipids.

Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.

Calcium-dependent protein kinase: widespread occurrence in various tissues and phyla of the animal kingdom and comparison of effects of phospholipid, calmodulin, and trifluoperazine.

Phosphorylated oligosaccharides in lysosomal enzymes: identification of alpha-N-acetylglucosamine(1)phospho(6)mannose diester groups.

Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance.

Oligosaccharide moieties of glycoprotein hormones: bovine lutropin resists enzymatic deglycosylation because of terminal O-sulfated N-acetylhexosamines.

Structure of the metal clusters in rabbit liver metallothionein

Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.

Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.

In vitro transcription of normal, mutant, and truncated mouse alpha-globin genes.

Electrophoretic resolution of three major insulin receptor structures with unique subunit stoichiometries.

Kinetic study of the interaction of oxy- and deoxyhemoglobins with the erythrocyte membrane.

Model for lactose repressor protein and its interaction with ligands.

Adenylate deaminase binding to synthetic thick filaments of myosin.

Covalent binding and hemolytic activity of complement proteins.

Molecular structure of an anticancer drug-DNA complex: daunomycin plus d(CpGpTpApCpG).

Kinetic studies of the cooperative binding of subfragment 1 to regulated actin.

Isolation and amino acid sequence of gamma-lipotropin from human pituitary glands.

Electron tunneling process and the segment mobility of macromolecules.

Nature of the open state in long polynucleotide double helices: possibility of soliton excitations.

Magneto-orientation of lecithin crystals.

A new approach to time-resolved studies of ATP-requiring biological systems; laser flash photolysis of caged ATP.

Receptor-mediated transcellular transport of immunoglobulin: synthesis of secretory component as multiple and larger transmembrane forms.

Relationship between x-ray exposure and malignant transformation in C3H 10T1/2 cells.

Probabilistic view of the transformation of cultured C3H/10T1/2 mouse embryo fibroblasts by 3-methylcholanthrene.

Enhancement of N-methyl-N'-nitro-N-nitrosoguanidine transformation of Syrian hamster cells by a phorbol diester is independent of sister chromatid exchanges and chromosome aberrations.

Biochemical characterization of human melanoma cell surfaces: dissection with monoclonal antibodies.

Nucleation and the kinetics of microtubule assembly.

Radiosensitivity in ataxia-telangiectasia: a new explanation.

Fibronectin is not present in the focal adhesions formed between normal cultured fibroblasts and their substrata.

Nucleotide sequence divergence and functional constraint in mRNA evolution.

Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii.

Cell cycle-specific mutagenesis at the hypoxanthine phosphoribosyltransferase locus in adult rat liver epithelial cells.

Noninvasive, nondestructive approaches to cell bioenergetics.

In vitro gamma irradiation of leukemic cells in mice, rats, and guinea pigs.

Lipid fluidity markedly modulates the binding of serotonin to mouse brain membranes.

Release of enkephalins and enkephalin-containing polypeptides from perfused beef adrenal glands.

Bicuculline enhances a negative component and diminishes a positive component of the visual evoked cortical potential in the cat.

Current recorded from a cut-open giant axon under voltage clamp.

Deoxycorticosterone biosynthesis in human kidney: potential for formation of a potent mineralocorticosteroid in its site of action.

Cell cycle and the differential expression of HLA-A,B and HLA-DR antigens on human B lymphoid cells.

Characterization of a protease apparently involved in processing of pre-ornithine transcarbamylase of rat liver.

Feedback regulation of ribosomal protein gene expression in Escherichia coli: structural homology of ribosomal RNA and ribosomal protein MRNA.

Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.

Inhibition of biological activity of multiplication-stimulating activity by binding to its carrier protein.

Replication of plasmids from Staphylococcus aureus in Escherichia coli.

Inversions of specific DNA segments in flagellar phase variation of Salmonella and inversion systems of bacteriophages P1 and Mu.

Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

In vivo germinal mutation detection with 'monospecific' antibody against lactate dehydrogenase-X.

Insulin release, insulin sensitivity, and glucose intolerance.

Preparation of antibodies specific to choline acetyltransferase from bovine caudate nucleus and immunohistochemical localization of the enzyme.

National Academy of Sciences October 1, 1980.

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Novel RNA polymerase sigma factor from Bacillus subtilis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Haldenwang, W. G. Losick, R.
Abstract	A modified form of Bacillus subtilis RNA polymerase (RNA nucleotidyltransferase) has been isolated that exhibits distinctive transcriptional specificity. This modified enzyme transcribes two cloned genes from the purA-cysA region of the B. subtilis chromosome whose expression in vivo is associated with the process of sporulation. Neither of these genes is transcribed by the usual form of B. subtilis RNA polymerase holoenzyme containing a sigma factor of 55,000 daltons (sigma 55). The modified RNA polymerase lacks sigma 55 but contains a newly identified subunit of 37,000 daltons termed sigma 37. A reconstitution experiment in which sigma 37 was added to core RNA polymerase strongly suggests that sigma 37 is responsible for the transcriptional specificity of the modified RNA polymerase. Sigma 37 apparently acts at the level of promoter recognition; this transcriptional determinant enabled core RNA polymerase to form stable binary and ternary ('initiation') complexes with endonuclease restriction fragments containing promoters for the cloned B. subtilis genes.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	12
Volume Number	77
Language	English
Publisher	National Academy of Sciences
Publisher Date	1980-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacillus Subtilis Enzymology Sigma Factor Physiology Transcription Factors Transcription, Genetic Genetics DNA, Bacterial DNA-Directed RNA Polymerases Metabolism Gene Expression Regulation Operon Protein Binding Isolation & Purification Spores, Bacterial Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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