NDLI: Reiteration frequency of immunoglobulin light chain genes: further evidence for somatic generation of antibody diversity.

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Reiteration frequency of immunoglobulin light chain genes: further evidence for somatic generation of antibody diversity.

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Reiteration frequency of immunoglobulin light chain genes: further evidence for somatic generation of antibody diversity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Tonegawa, S.
Abstract	Methods have been developed for preparing mouse immunoglobulin light chain mRNA of better than 90% purity. Hybridization of both lambda and kappa mRNAs to excess liver DNA yielded results compatible with gene reiteration frequencies of two to three. There was no evidence of hybridization of these highly purified mRNAs to reiterated DNA, and, in fact, the kinetics of hybridization were very similar to that of purified globin mRNA. Purified lambda mRNA from tumors producing structurally different lambda chains were used in competition hybridization experiments. An unlabeled lambda mRNA competed with another, labeled lambda mRNA to the same extent as homologous unlabeled lambda mRNA. That is, base sequence homology among lambda mRNAs is so high that any lambda mRNA should cross-hybridize with all germ line variable (Vlambda) genes at least for those V-regions which are represented among myelomas. From amino-acid sequence data, it is argued that there are probably more than 25 different lambda V regions. Hence it is concluded that the number of germ line genes is too small to account for the diversity of lambda chains. A similar conclusion is drawn for kappa chains.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	73
Language	English
Publisher	National Academy of Sciences
Publisher Date	1976-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antibody Formation Genes Immunoglobulin Light Chains Biosynthesis Antibody Specificity Cell Line Immunoglobulin Kappa-Chains Immunoglobulin Lambda-Chains Kinetics Nucleic Acid Hybridization RNA, Messenger Isolation & Purification Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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