NDLI: Extracellular labeling of nascent polypeptides traversing the membrane of Escherichia coli.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1977 Volume: 74 Issue: 12

Year: 1977 Volume: 74 Issue: 11

Year: 1977 Volume: 74 Issue: 10

Year: 1977 Volume: 74 Issue: 9

Year: 1977 Volume: 74 Issue: 8

Year: 1977 Volume: 74 Issue: 7

Escherichia coli 5S RNA binding proteins L18 and L25 interact with 5.8S RNA but not with 5S RNA from yeast ribosomes.

Sugar uptake into brush border vesicles from normal human kidney.

Model for 'charge-relay': acceleration by carboxylate anion in intramolecular general base-catalyzed ester hydrolysis by the imidazolyl group.

Superiority of interconvertible enzyme cascades in metabolite regulation: analysis of multicyclic systems.

Defective organization of actin in cultured skin fibroblasts from patients with inherited adenocarcinoma.

A protonmotive force drives bacterial flagella.

Electrical stimulation of preganglionic nerve increases tyrosine hydroxylase activity in sympathetic ganglia.

Excited state lifetimes in cytochromes measured from Raman scattering data: evidence for iron-porphyrin interactions.

Structural adaptations of lactate dehydrogenase isozymes.

Polypeptides of cells transformed by RNA or DNA tumor viruses.

Importance of 5'-terminal blocking structure to stabilize mRNA in eukaryotic protein synthesis.

Binding of Zn2+ to rat liver fructose-1,6-bisphosphatase and its effect on the catalytic properties.

Crosslinked histone octamer as a model of the nucleosome core.

Histones: metabolism in simian virus 40-infected cells and incorporation into virions.

Topographic separation of adenylate cyclase and hormone receptors in the plasma membrane of toad erythrocyte ghosts.

Glucocorticoid-stimulated accumulation of mouse mammary tumor virus RNA: increased rate of synthesis of viral RNA.

Control of growth by picolinic acid: differential response of normal and transformed cells.

Specific changes in the collagen phenotype of BALB 3T3 cells as a result of transformation by sarcoma viruses or a chemical carcinogen.

Segregation of genetic information for a B-tropic leukemia virus with the structural locus for BALB:virus-1.

Specific action of T4 endonuclease V on damaged DNA in xeroderma pigmentosum cells in vivo.

Suppressor cell regulation of immune response to tumors: abrogation by adult thymectomy.

Common precursor to corticotropins and endorphins.

Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

Isolation of transforming murine leukemia viruses from mice with a high incidence of spontaneous lymphoma.

Arrangement of water molecules in cavities and channels of the lattice of [Phe4Val6]antamanide dodecahydrate.

Magnetic properties and structure of oxyhemoglobin.

Kinetics of hemoglobin-carbon monoxide reactions measured with a superconducting magnetometer: a new method for fast reactions in solution.

Sequence specificity of mutagen-nucleic acid complexes in solution: intercalation and mutagen-base pair overlap geometries for proflavine binding to dC-dC-dG-dG and dG-dG-dC-dC self-complementary duplexes.

Structures of enzyme-substrate complexes of lysozyme.

Ultraviolet resonant Raman spectroscopy of nucleic acid components.

Molecular properties of the nerve growth factor secreted in mouse saliva.

Loss of infectivity of brome mosaic virus RNA after chemical modification of the 3' or 5' terminus.

A site-specific single-strand endonuclease from the eukaryote Chlamydomonas.

Biotinylinsulins as potential tools for receptor studies.

Interaction between the third complement protein and cell surface macromolecules.

A DNA fragment containing the origin of replication of the Escherichia coli chromosome.

Distribution of 5-methylcytosine in chromatin.

Enzymes as reagents in peptide synthesis: enzyme-labile protection for carboxyl groups.

Glycophorin and the concanavalin A receptor of human erythrocytes: their receptor function in lipid bilayers.

Superiority of interconvertible enzyme cascades in metabolic regulation: analysis of monocyclic systems.

Concept of internal structural controls for evaluation of inactive synthetic peptide analogs: synthesis of [Orn13,14]apamin and its guanidination to an apamin derivative with full neurotoxic activity.

Discontinuous replication of replicative form DNA from bacteriophage phiX174.

Nerve growth factor receptors: identification of distinct classes in plasma membranes and nuclei of embryonic dorsal root neurons.

Isolation and identification of aliphatic short-chain acylcarnitines from beef heart: possible role for carnitine in branched-chain amino acid metabolism.

Molecular weight of bacteriorhodopsin solubilized in Triton X-100.

Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes.

DNA or RNA priming of bacteriophage G4 DNA synthesis by Escherichia coli dnaG protein.

Structural convergence during protein evolution.

Method for obtaining a high resolution protein map starting from a low resolution map.

The living state and cancer.

Extensive disulfide bonding at the mammalian cell surface.

Effects of antitubulins on the redistribution of crosslinked receptors on the surface of fibroblasts and epithelial cells.

Regulation of DNA synthesis: age-dependent cooperation among G1 cells upon fusion.

Inhibition of cytokinesis in Friend leukemia cells by membrane mobility agents.

Tumor promoters inhibit spontaneous differentiation of Friend erythroleukemia cells in culture.

Independent expression of the adrenergic phenotype by neural crest cells in vitro.

Food, drug, and cosmetic dyes: biological effects related to lipid solubility.

Hemoglobin F synthesis in vitro: evidence for control at the level of primitive erythroid stem cells.

Epigenetic variation of cultured somatic cells: evidence for gradual changes in the requirement for factors promoting cell division.

Serial transfer of a human gene to rodent cells by sequential chromosome-mediated gene transfer.

Detection of active heteropolymeric beta-glucuronidase in hybrids between mouse cells and human fibroblasts with beta-glucuronidase deficiency.

Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.

Glycolipid-bound sialic acid in serum: increased levels in mice and humans bearing mammary carcinomas.

Isoantigenic expression of Forssman glycolipid in human gastric and colonic mucosa: Its possible identity with “A-like antigen” in human cancer

In vitro and in vivo correlation of clotting protease activity: effect of heparin.

Cholecystokinin and its COOH-terminal octapeptide in the pig brain.

Fatty liver induced in Zucker 'fatty' (ff) rats by a semisynthetic diet rich in sucrose. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 74 Issue: 7)

Measurement of daily cholesterol synthesis rates in man by assay of the fractional conversion of mevalonic acid to cholesterol.

Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia.

Variation in ability of measles virus plaque progeny to induce interferon.

Nonlinear analysis of cat retinal ganglion cells in the frequency domain.

Modulation of acetylcholine receptor by antibody against the receptor.

Lithium efflux through the Na/K pump in human erythrocytes.

Specific radiolabeling of a cell surface receptor for epidermal growth factor.

Reinnervation of original synaptic sites on muscle fiber basement membrane after disruption of the muscle cells.

Mannosidosis: assignment of the lysosomal alpha-mannosidase B gene to chromosome 19 in man.

Interaction of rabbit reticulocyte ribosomes with bacteriophage f1 mRNA and of Escherichia coli ribosomes with rabbit globin mRNA.

Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli.

Expression of petite mitochondrial DNA in vivo: zygotic gene rescue.

Mutagenicity and cytotoxicity of benz[alpha]anthracene diol epoxides and tetrahydro-epoxides: exceptional activity of the bay region 1,2-epoxides.

Release of Escherichia coli DNA from membrane complexes by single-strand endonucleases.

Extracellular labeling of nascent polypeptides traversing the membrane of Escherichia coli.

Isolation of folded chromosomes from the yeast Saccharomyces cerevisiae.

Protease effects on specific growth properties of normal and transformed baby hamster kidney cells.

Mobility and distribution of a cell surface glycoprotein and its interaction with other membrane components.

Recombinant plasmid that carries part of the nitrogen fixation (nif) gene cluster of Klebsiella pneumoniae.

Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity.

Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12.

Rationale for combined use of fetal liver and thymus for immunological reconstitution in patients with variants of severe combined immunodeficiency.

Immunohistochemical localization in rabbit brain of a peptide resembling the COOH-terminal octapeptide of cholecystokinin.

Immunohistochemical analysis of peptide pathways possibly related to pain and analgesia: enkephalin and substance P.

Serotonergic neurons in the peripheral nervous system: identification in gut by immunohistochemical localization of tryptophan hydroxylase.

Genetic instability in Drosophila melanogaster: putative multiple insertion mutants at the singed bristle locus.

Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.

Dimeric immunoglobulin E serves as a unit signal for mast cell degranulation.

Cleavage of C2 by C1s into the antigenically distinct fragments C2a and C2b: demonstration of binding of C2b to C4b.

Specificity studies on cytotoxic thymus-derived lymphocytes reactive with influenza virus-infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens.

Year: 1977 Volume: 74 Issue: 6

Year: 1977 Volume: 74 Issue: 5

Year: 1977 Volume: 74 Issue: 4

Year: 1977 Volume: 74 Issue: 3

Year: 1977 Volume: 74 Issue: 2

Year: 1977 Volume: 74 Issue: 1

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Extracellular labeling of nascent polypeptides traversing the membrane of Escherichia coli.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Davis, B. D. Smith, W. P. Tai, P. C. Thompson, R. C.
Abstract	To provide direct evidence for the hypothesis that secreted proteins may traverse membranes as growing chains, we labeled spheroplasts of Escherichia coli with a reagent (acetyl[35S]methionyl methylphosphate sulfone) that reacts with amino groups but does not cross the membrane. After fractionation, about 6% of the label in the membrane-polysome fraction was found to be attached to the polysomes. This attachment was via peptidyl-tRNA, as shown by several tests: release of most of the label from purified polysomes at low Mg2+; subsequent loss of about 25,000 daltons on cleavage by dilute alkali; release by puromycin; and release, accompanied by a marked increase in average molecular weight, on peptide chain completion. Moreover, a significant fraction of the completed chains was identified serologically and by molecular weight as a major periplasmic protein, alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1]. This work provides direct evidence that: (i) secreted proteins thread through the membrane as growing peptide chains; and (ii) membrane-associated polysomes in bacteria are functionally attached to membrane and not merely trapped on disruption of the cell.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	74
Language	English
Publisher	National Academy of Sciences
Publisher Date	1977-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacterial Proteins Secretion Escherichia Coli Metabolism Polyribosomes Alkaline Phosphatase Biosynthesis Cell Membrane Enzyme Precursors RNA, Transfer Sulfones Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in