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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Colcher, D. Teramoto, Y. A. Schlom, J. |
| Abstract | A competition radioimmunoassay has been developed in which type-D retroviruses from three primate species compete. The assay utilizes the major structural protein (36,000 daltons) of the endogenous squirrel monkey retrovirus and antisera directed against the major structural protein (27,000 daltons) of the Mason-Pfizer monkey virus isolated from rhesus monkeys. Purified preparations of both viruses grown in heterologous cells, as well as extracts of heterologous cells infected with squirrel monkey retrovirus or Mason-Pfizer monkey virus, compete completely in the assay. Addition of an endogenous virus of the langur monkey also results in complete blocking. No blocking in the assay is observed with type-C baboon viruses, woolly monkey virus, and gibbon virus. Various other type-C and type-B viruses also showed no reactivity. An interspecies assay has thus been developed that recognizes the type-D retroviruses from both Old World monkey (rhesus and langur) and New World monkey (squirrel) species. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 12 |
| Volume Number | 74 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1977-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Haplorhini Microbiology Retroviridae Immunology Viral Proteins Animals Binding, Competitive Cell Line Glycoproteins Molecular Weight Radioimmunoassay Species Specificity Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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