NDLI: Increased expression of a eukaryotic gene in Escherichia coli through stabilization of its messenger RNA.

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Increased expression of a eukaryotic gene in Escherichia coli through stabilization of its messenger RNA.

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Increased expression of a eukaryotic gene in Escherichia coli through stabilization of its messenger RNA.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Giles, N. H. Bassett, C. L. Kushner, S. R. Hautala, J. A.
Abstract	The expression of a cloned eukaryotic gene [catabolic dehydroquinase (3-dehydroquinate hydro-lyase, EC 4.2.1.10) (qa-2+) from Neurospora crassa] is dramatically increased (as much as 100-fold) in Escherichia coli strains deficient in polynucleotide phosphorylase (pnp) (polynucleotide: orthophosphate nucleotidyltransferase, EC 2.7.7.8) and RNase I (rna). The increased expression is controlled primarily by the absence of polynucleotide phosphorylase and appears to be specific for the eukaryotic gene. No increase in the specific activity of either chromosomal or plasmid-borne prokaryotic genes has been observed. In polynucleotide phosphorylase-deficient strains of E. coli the half-life of plasmid (pVK88, ampr qa-2+)-encoded mRNAs increases from 1.0 to 2.8 min. This increase must be due primarily to stabilization of the aq-2 mRNA because no increase in the half-lives of pBR322 vehicle mRNAs was observed in polynucleotide phosphorylase-deficient strains. These results suggest that there are inherent structural differences between prokaryotic and eukaryotic mRNAs.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	11
Volume Number	76
Language	English
Publisher	National Academy of Sciences
Publisher Date	1979-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA, Recombinant Escherichia Coli Genetics Hydro-Lyases RNA, Messenger Metabolism Enzymology Genes Neurospora Crassa Plasmids Polyribonucleotide Nucleotidyltransferase Quinic Acid Analogs & Derivatives Ribonucleases Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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