NDLI: Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment whose transcription is regulated by glucocorticoids in vivo.

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Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment whose transcription is regulated by glucocorticoids in vivo.

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Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment whose transcription is regulated by glucocorticoids in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Carlstedt-duke, J. Gustafsson, J. A. Payvar, F. Wrange, O. Okret, S. Yamamoto, K. R.
Abstract	Activated glucocorticoid receptor protein, purified to 40-60% homogeneity from rat liver extracts, binds selectively in vitro to a cloned fragment of murine mammary tumor virus (MTV) DNA. The DNA fragment tested contains about half of the sequences present in intact MTV DNA, and its rate of transcription, like that of the intact viral element, is strongly stimulated by glucocorticoids when it is introduced into the genome of a receptor-containing cell. In contrast, the receptor fails to bind selectively to DNA restriction fragments from E. coli plasmids pBR322 and RSF2124 or from bacteriophages lambda and T4. Preliminary experiments to localize regions within MTV DNA responsible for selective binding have revealed thus far one subfragment that fails to bind the receptor and one selectively bound subfragment that maps far downstream from the 5' terminus of the normal RNA transcript. These studies are consistent with the notion that steroid receptors may modulate rates of transcription by recognizing specific DNA sequences within or near the regulated genes.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	11
Volume Number	78
Language	English
Publisher	National Academy of Sciences
Publisher Date	1981-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cloning, Molecular DNA, Recombinant Metabolism Glucocorticoids Physiology Liver Receptors, Glucocorticoid Receptors, Steroid Transcription, Genetic Animals Bacteriophage Lambda Genetics DNA Restriction Enzymes DNA, Viral Mammary Tumor Virus, Mouse Molecular Weight Isolation & Purification Drug Effects Triamcinolone Acetonide Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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