NDLI: The tac promoter: a functional hybrid derived from the trp and lac promoters.

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The tac promoter: a functional hybrid derived from the trp and lac promoters.

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The tac promoter: a functional hybrid derived from the trp and lac promoters.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Comstock, L. J. De Boer, H. A. Vasser, M.
Abstract	Two hybrid promoters that are functional in Escherichia coli have been constructed. These hybrid promoters, tacI and tacII, were derived from sequences of the trp and the lac UV5 promoters. In the first hybrid promoter (tacI), the DNA upstream of position -20 with respect to the transcriptional start site was derived from the trp promoter. The DNA downstream of position -20 was derived from the lac UV5 promoter. In the second hybrid promoter (tacII), the DNA upstream of position -11 at the Hpa I site within the Pribnow box was derived from the trp promoter. The DNA downstream of position -11 is a 46-base-pair synthetic DNA fragment that specifies part of the hybrid Pribnow box and the entire lac operator. It also specifies a Shine-Dalgarno sequence flanked by two unique restriction sites (portable Shine-Dalgarno sequence). The tacI and the tacII promoters respectively direct transcription approximately 11 and 7 times more efficiently than the derepressed parental lac UV5 promoter and approximately 3 and 2 times more efficiently than the trp promoter in the absence of the trp repressor. Both hybrid promoters can be repressed by the lac repressor and both can be derepressed with isopropyl beta-D-thiogalactoside. Consequently, these hybrid promoters are useful for the controlled expression of foreign genes at high levels in E. coli. In contrast to the trp and the lac UV5 promoters, the tacI promoter has not only a consensus -35 sequence but also a consensus Pribnow box sequence. This may explain the higher efficiency of this hybrid promoter with respect to either one of the parental promoters.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	80
Language	English
Publisher	National Academy of Sciences
Publisher Date	1983-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Lac Operon Operon Tryptophan Genetics Escherichia Coli Gene Expression Regulation Genetic Engineering Plasmids Structure-Activity Relationship Transcription, Genetic Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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