NDLI: Spontaneous activation of a human proto-oncogene.

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Radioprotection of human lymphoid cells by exogenously supplied glutathione is mediated by gamma-glutamyl transpeptidase.

Immunocytochemical localization of gamma-glutamyltransferase in induced hyperplastic nodules of rat liver.

Primary structures of human alpha-fetoprotein and its mRNA.

Nucleotide sequence of the cDNA encoding the precursor of the beta subunit of rat lutropin.

A precise termination site in the mouse beta major-globin transcription unit.

T-cell hybridoma specific for a cytochrome c peptide: specific antigen binding and interleukin 2 production.

Treatment of sickle cell anemia with 5-azacytidine results in increased fetal hemoglobin production and is associated with nonrandom hypomethylation of DNA around the gamma-delta-beta-globin gene complex.

Phenomenon of formation of giant fat-containing cells in human bone marrow cultures induced by human serum factor: normal and leukemic patterns.

Importance of secondary structure in the signal sequence for protein secretion.

(2'-5')Oligoadenylate in rat liver: modulation after partial hepatectomy.

Comparison of sea urchin and human mtDNA: evolutionary rearrangement.

The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.

Tubulin genes of Trypanosoma brucei: a tightly clustered family of alternating genes.

Methyl-directed repair of DNA base-pair mismatches in vitro.

ts A1S9 locus in mouse L cells may encode a novobiocin binding protein that is required for DNA topoisomerase II activity.

Expression and characterization of the product of a human immune interferon cDNA gene in Chinese hamster ovary cells.

Identification of Escherichia coli DNA helicase I as the traI gene product of the F sex factor.

DNA stem-loop structures bind poorly to histone octamer cores.

Mössbauer and EPR studies of activated aconitase: development of a localized valence state at a subsite of the [4Fe-4S] cluster on binding of citrate.

Spontaneous activation of a human proto-oncogene.

Transforming growth factors produced by retrovirus-transformed rodent fibroblasts and human melanoma cells: amino acid sequence homology with epidermal growth factor.

Methylation status and DNase I sensitivity of immunoglobulin genes: changes associated with rearrangement.

Enhanced synthesis and stabilization of Mr 68,000 protein in transformed BALB/c-3T3 cells: candidate for restriction point control of cell growth.

Loss of leukemogenicity caused by mutations in the membrane glycoprotein structural gene of Friend spleen focus-forming virus.

A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT.

Envelope gene of the Friend spleen focus-forming virus: deletion and insertions in 3' gp70/p15E-encoding region have resulted in unique features in the primary structure of its protein product.

Cyclic AMP-dependent constitutive expression of gal operon: use of repressor titration to isolate operator mutations.

Mini-F plasmid genes that couple host cell division to plasmid proliferation.

Two small RNAs encoded by Epstein-Barr virus can functionally substitute for the virus-associated RNAs in the lytic growth of adenovirus 5.

Localization of c-ras oncogene family on human germ-line chromosomes.

Expression of bacterial genes in plant cells.

Rearrangement and amplification of c-abl sequences in the human chronic myelogenous leukemia cell line K-562.

A DNA repair gene required for the incision of damaged DNA is essential for viability in Saccharomyces cerevisiae.

Differential expression of the normal and of the translocated human c-myc oncogenes in B cells.

Hepatitis B virus DNA in Kaposi sarcoma.

Molecular requirements for B-lymphocyte activation by Escherichia coli lipopolysaccharide.

Recombination within and between the human insulin and beta-globin gene loci.

Pancreatic epithelium is permeable to sucrose and inulin across secretory cells.

Alpha-pyridine nucleotides as substrates for a plasmid-specified dihydrofolate reductase.

Maximum-entropy calculation of the electron density at 4 A resolution of Pf1 filamentous bacteriophage.

Biochemical and molecular analysis of naturally occurring Adh variants in Drosophila melanogaster.

Amino acid sequence of the Fv region of a human monoclonal IgM (protein WEA) with antibody activity against 3,4-pyruvylated galactose in Klebsiella polysaccharides K30 and K33.

ADP-ribosylation of the Mr 83,000 stress-inducible and glucose-regulated protein in avian and mammalian cells: modulation by heat shock and glucose starvation.

Increased membrane permeability to chloride in Duchenne muscular dystrophy fibroblasts and its relationship to muscle function.

'Prompt' heat shock proteins: translationally regulated synthesis of new proteins associated with the nuclear matrix-intermediate filaments as an early response to heat shock.

Interaction of fibronectin with collagen: age-specific defect in the biological activity of human fibroblast fibronectin.

Development of translationally active mRNA for larval muscle acetylcholinesterase during ascidian embryogenesis.

Three organizations of human DNA.

Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.

Defective myoblasts identified in Duchenne muscular dystrophy.

Viral nucleic acid synthesis and antigen accumulation in pancreas and kidney of Pekin ducks infected with duck hepatitis B virus.

Characterization of rat hypothalamic corticotropin-releasing factor.

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Spontaneous activation of a human proto-oncogene.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Santos, E. Reddy, E. P. Barbacid, M. Pulciani, S. Feldmann, R. J.
Abstract	It has been recently shown that malignant activation of the c-has/bas proto-oncogene in T24 human bladder carcinoma cells was mediated by a single point mutation. A deoxyguanosine located at position 35 of the first exon of this proto-oncogene was substituted by thymidine. These findings predicted that the resulting oncogene would code for a structurally altered p21 protein containing valine instead of glycine as its 12th amino acid residue. We now report the spontaneous activation of the human c-has/bas proto-oncogene during transfection of NIH/3T3 cells. As in T24 cells, this in vitro activated oncogene also acquired malignant properties by a single point mutation. In this case we have detected a G leads to A transition, which occurred at the same position as the mutation responsible for the activation of the T24 oncogene. These results predict that the p21 protein coded for by the spontaneously activated c-has/bas gene will incorporate aspartic acid as its 12th amino acid residue. Computer analysis of the secondary structure of c-has/bas encoded p21 proteins indicates that substitution of the glycine residue located at position 12, not only by aspartic acid or valine but also by any other amino acid, would result in the same structural alteration. These findings indicate that a specific conformational change is sufficient to confer transforming properties to this p21 protein. Moreover, they predict that any mutation affecting the coding properties of the 12th codon of the c-has/bas proto-oncogene will lead to its malignant activation.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	15
Volume Number	80
Language	English
Publisher	National Academy of Sciences
Publisher Date	1983-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Transformation, Neoplastic Oncogenes Urinary Bladder Neoplasms Genetics Amino Acid Sequence Animals Cell Line Cells, Cultured DNA Restriction Enzymes Mice Mutation Neoplasm Proteins Nucleic Acid Hybridization Phenotype Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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