NDLI: Specific DNA sequences associated with the nuclear matrix in synchronized mouse 3T3 cells.

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Biosynthesis of renin: multiplicity of active and intermediate forms.

Partial cDNA sequence to a hamster gene corrects defect in Escherichia coli pyrB mutant.

Inhibition of transcription in somatic cells by microinjection of antibodies to chromosomal proteins.

Selective cleavage in the avian retroviral long terminal repeat sequence by the endonuclease associated with the alpha beta form of avian reverse transcriptase.

Engineering of site-directed antisera against vertebrate calmodulin by using synthetic peptide immunogens containing an immunoreactive site.

Alteration and activation of sequence-specific cleavage of DNA by bleomycin in the presence of the antitumor drug cis-diamminedichloroplatinum(II).

Immunological crossreactivity of eukaryotic C1-tetrahydrofolate synthase and prokaryotic 10-formyltetrahydrofolate synthetase.

Polypeptides immunologically related to band 3 are present in nucleated somatic cells.

High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.

Different 3' end points of deletions causing delta beta-thalassemia and hereditary persistence of fetal hemoglobin: implications for the control of gamma-globin gene expression in man.

Stable low molecular weight DNA in xeroderma pigmentosum cells.

Genetic recombination of bacteriophage lambda DNAs in Xenopus oocytes.

Targeted selection of recombinant clones through gene dosage effects.

A specific phosphoprotein phosphatase acts on histone H1 phosphorylated by protein kinase C.

Identification of a transposon Tn3 sequence required for transposition immunity.

A perfectly symmetric lac operator binds the lac repressor very tightly.

Definition of oriR, the minimum DNA segment essential for initiation of R1 plasmid replication in vitro.

Affinity labeling and partial purification of nerve growth factor receptors from rat pheochromocytoma and human melanoma cells.

Structural similarities between the development-specific protein S from a gram-negative bacterium, Myxococcus xanthus, and calmodulin.

Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.

Nucleotide sequence of the structural gene for diphtheria toxin carried by corynebacteriophage beta.

Monoclonal antibodies that prevent adhesion of B 16 melanoma cells and reduce metastases in mice: crossreaction with human tumor cells.

Calmodulin-binding proteins that interact with actin filaments in a Ca2+-dependent flip-flop manner: survey in brain and secretory tissues.

Molecular mechanisms of human hemoglobin switching: selective undermethylation and expression of globin genes in embryonic, fetal, and adult erythroblasts.

Transformasomes: specialized membranous structures that protect DNA during Haemophilus transformation.

Mitochondrial DNA heteroplasmy in Drosophila mauritiana.

Molecular map of the murine S region.

Restriction fragment length polymorphisms associated with immunoglobulin C gamma genes reveal linkage disequilibrium and genomic organization.

Hybrid gamma 2b-gamma 2a genes expressed in myeloma variants: evidence for homologous recombination.

Monoclonal antibodies against the antigen receptor on a cloned T-cell hybrid.

Glucocorticoids regulate proopiomelanocortin gene expression in vivo at the levels of transcription and secretion.

Human T-cell leukemia virus type II transforms normal human lymphocytes.

Anticodon loop size and sequence requirements for recognition of formylmethionine tRNA by methionyl-tRNA synthetase.

Location of eight ribosomal proteins on the surface of the 50S subunit from Escherichia coli.

ATP-dependent protein kinase-catalyzed phosphorylation of a seryl residue in HPr, a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes.

Thermodynamics of assembly of Escherichia coli aspartate transcarbamoylase.

Gene conversion and associated reciprocal recombination are separable events in vegetative cells of Saccharomyces cerevisiae.

Carbon starvation and growth rate-dependent regulation of the Escherichia coli ribosomal RNA promoters: differential control of dual promoters.

Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA X Fe(II).

Activation of the heme-stabilized translational inhibitor of reticulocyte lysates by calcium ions and phospholipid.

Purified rat brain calcium- and phospholipid-dependent protein kinase phosphorylates ribosomal protein S6.

Autogenous production of a hemopoietic growth factor, persisting-cell-stimulating factor, as a mechanism for transformation of bone marrow-derived cells.

Transcriptional activation of an unrearranged and untranslocated c-myc oncogene by translocation of a C lambda locus in Burkitt.

Characterization of the human receptor for T-cell growth factor.

Electrophoretic analysis of human HLA-DR antigens from HLA-DR4 homozygous cell lines: correlation between beta-chain diversity and HLA-D.

A monoclonal anti-platelet antibody with decreased reactivity for autoimmune thrombocytopenic platelets.

Correlation between rate constant for reduction and redox potential as a basis for systematic investigation of reaction mechanisms of electron transfer proteins.

Latent tissue plasminogen activator produced by human endothelial cells in culture: evidence for an enzyme-inhibitor complex.

Isolation of a genomic clone for bovine pancreatic trypsin inhibitor by using a unique-sequence synthetic DNA probe.

Plasma membrane retrieval in neoplastic pancreatic acinar cells.

Specific DNA sequences associated with the nuclear matrix in synchronized mouse 3T3 cells.

Arylsulfatase in natural killer cells: its possible role in cytotoxicity.

Hemagglutinin of swine influenza virus: a single amino acid change pleiotropically affects viral antigenicity and replication.

Prothrombin is activated on vascular endothelial cells by factor Xa and calcium.

Use of a monoclonal antibody as a substrate for mature neurons in vitro.

Lipid metabolism of isolated oligodendrocytes maintained in long-term culture mimics events associated with myelinogenesis.

Comparative risk analysis of technological hazards (a review).

Oxygenation of hydrocarbons by cytochrome P-450 model compounds: modification of reactivity by axial ligands.

Resonance Raman studies of nitric oxide binding to ferric and ferrous hemoproteins: detection of Fe(III)--NO stretching, Fe(III)--N--O bending, and Fe(II)--N--O bending vibrations.

Structural homology of corticotropin-releasing factor, sauvagine, and urotensin I: circular dichroism and prediction studies.

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Specific DNA sequences associated with the nuclear matrix in synchronized mouse 3T3 cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Collier, I. Cassel, A. Goldberg, G. I.
Abstract	Eukaryotic chromatin appears to be organized into arrays of supercoiled loops anchored to the scaffolding structure of the mitotic chromosome core or to the nuclear matrix of interphase nuclei. To reveal whether specific DNA sequences are involved in this level of chromatin organization, we isolated and cloned a population of DNA molecules [average length of 150 base pairs (bp)] closely associated with the nuclear matrix after exhaustive DNase digestion and subsequent extensive protease digestion. The nuclear matrix was obtained from murine BALB/c 3T3 cells synchronized at the G1/S border of the cell cycle. We report the structure of two sequences, designated G4 and G5, which are highly enriched in the matrix DNA. Sequence G4, of 152 bp, contains three 31-bp direct head-to-tail repeats. An 11-bp sequence at the end of each repeat is homologous to the first large tumor antigen recognition site of human papova virus. Sequence G5, of 135 bp, consists of two well-defined domains, in which the first domain is a fragment of the B1 repetitive sequence. The results suggest the possibility that the loops of histone-depleted chromatin are connected to the scaffold of the nuclear matrix, with specific DNA sequences at the anchorage sites.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	22
Volume Number	80
Language	English
Publisher	National Academy of Sciences
Publisher Date	1983-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Nucleus Physiology Cloning, Molecular DNA Genetics Animals Cell Cycle Cells, Cultured DNA, Recombinant Kinetics Mice Mice, Inbred BALB C Nucleic Acid Conformation Nucleic Acid Hybridization Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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