NDLI: The retrovirus pol gene encodes a product required for DNA integration: identification of a retrovirus int locus.

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The retrovirus pol gene encodes a product required for DNA integration: identification of a retrovirus int locus.

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The retrovirus pol gene encodes a product required for DNA integration: identification of a retrovirus int locus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Temin, H. M. Panganiban, A. T.
Abstract	We mutagenized cloned spleen necrosis virus DNA to identify a region of the retrovirus genome encoding a polypeptide required for integration of viral DNA. Five plasmids bearing different lesions in the 3' end of the pol gene were examined for the ability to integrate or replicate following transfection of chicken embryo fibroblasts. Transfection with one of these DNAs resulted in the generation of mutant virus incapable of integrating but able to replicate at low levels; this phenotype is identical to that of mutants bearing alterations in the cis-acting region, att. To determine whether the 3' end of the pol gene encodes a protein that interacts with att, we did a complementation experiment. Cells were first infected with an att- virus and then superinfected with the integration-deficient virus containing a lesion in the pol gene and a wild-type att site. The results showed that the att- virus provided a transacting function allowing integration of viral DNA derived from the mutant bearing a wild-type att site. Thus, the 3' end of the pol gene serves as an 'int' locus and encodes a protein mediating integration of retrovirus DNA through interaction with att.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	24
Volume Number	81
Language	English
Publisher	National Academy of Sciences
Publisher Date	1984-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Transformation, Viral DNA, Viral Genetics Genes Retroviridae Animals Chick Embryo DNA Replication DNA Restriction Enzymes Fibroblasts Genetic Complementation Test Genotype Mutation Plasmids RNA-Directed DNA Polymerase Metabolism Transfection Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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