NDLI: A partial cDNA clone for human apolipoprotein B.

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A partial cDNA clone for human apolipoprotein B.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Deeb, S. S. Motulsky, A. G. Albers, J. J.
Abstract	A human liver cDNA library was screened for sequences coding for apolipoprotein B (apo B), the major protein of human low density lipoproteins. A mixture of synthetic oligonucleotides (26 bases long) coding for an amino acid sequence known to exist in apo B was used as a hybridization probe. A clone was identified that had a cDNA insert of 593 base pairs and that contained sequences coding for a peptide of 24 residues that had earlier been isolated from apo B by limited proteolysis. The entire nucleotide sequence of the cDNA insert consists of one open reading frame coding for 197 amino acids. Apo B-related RNAs were found in human liver, baboon liver, and the human hepatoma cell line HepG2. None were detected in placenta, simian virus 40 (SV40)-transformed fibroblasts, and a lymphoblastoid cell line. The length of the mature apo B mRNA was estimated to be 18 kb, enough to code for a protein with a molecular weight in the neighborhood of 500,000.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	15
Volume Number	82
Language	English
Publisher	National Academy of Sciences
Publisher Date	1985-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Apolipoproteins B Genetics Amino Acid Sequence Animals Cloning, Molecular DNA Liver RNA, Messenger Comparative Study Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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