NDLI: Molecular cloning and regulated expression of the human c-myc gene in Escherichia coli and Saccharomyces cerevisiae: comparison of the protein products.

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Molecular cloning and regulated expression of the human c-myc gene in Escherichia coli and Saccharomyces cerevisiae: comparison of the protein products.

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Molecular cloning and regulated expression of the human c-myc gene in Escherichia coli and Saccharomyces cerevisiae: comparison of the protein products.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rupprecht, K. Kumar, G. Chizzonite, R. Miyamoto, C. Schaber, M. Poonian, M. Kramer, R. Crowl, R. Ju, G.
Abstract	mRNA from human HL-60 cells was used to prepare a cDNA library, from which two full-length clones that encompass the complete c-myc coding region were isolated. One clone, pM1-11, contains all three exons of human c-myc. The second clone, pM4-10, represents a relatively rare transcript that initiated in the first intron and includes the coding exons 2 and 3. The cDNA insert in pM1-11 was used to express the human c-myc protein in both prokaryotic and eukaryotic cells. Insertion of the coding sequences in exons 2 and 3 into the appropriate expression vectors yielded detectable c-myc protein in Escherichia coli lacking the Lon protease and in Saccharomyces cerevisiae upon induction. The protein produced in E. coli has an apparent size of 60 kDa and appears to be unmodified, as it is identical in size to the protein synthesized in an in vitro system. In contrast, yeast cells synthesize two myc proteins, of 60 kDa and 62 kDa. The difference in apparent molecular mass between the two proteins appears to be due, in part, to phosphorylation. Subcellular fractionation of yeast cells showed that the c-myc phosphoprotein is located predominantly in the nuclear fraction.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	21
Volume Number	82
Language	English
Publisher	National Academy of Sciences
Publisher Date	1985-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Escherichia Coli Genetics Gene Expression Regulation Neoplasm Proteins Biosynthesis Proto-Oncogene Proteins Proto-Oncogenes Recombinant Proteins Metabolism Saccharomyces Cerevisiae Cell Line Cloning, Molecular DNA Leukemia, Myeloid Phosphorylation Species Specificity Transcription, Genetic Comparative Study Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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